肺抗原呈递细胞：分离、纯化及培养
Pulmonary antigen presenting cells: isolation, purification, and culture.
作者信息
Nakano Hideki, Cook Donald N
机构信息
Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
出版信息
Methods Mol Biol. 2013;1032:19-29. doi: 10.1007/978-1-62703-496-8_2.
Abstract
Antigen presenting cells (APCs) such as dendritic cells (DCs) and macrophages comprise a relatively small fraction of leukocytes residing in lymphoid and non-lymphoid tissues. Accordingly, functional analyses of these cells have been hampered by low cell yields. Also, alveolar macrophages share several physical properties with DCs, and this has complicated efforts to prepare pure populations of lung APCs. To overcome these difficulties, we have developed improved flow cytometry-based methods to analyze and purify APCs from the lung and its draining lymph nodes (LNs). In this chapter, we describe these methods in detail, as well as methods for culturing APCs and characterizing their interactions with T cells.
摘要
抗原呈递细胞（APC），如树突状细胞（DC）和巨噬细胞，在驻留在淋巴组织和非淋巴组织中的白细胞中所占比例相对较小。因此，这些细胞的功能分析因细胞产量低而受到阻碍。此外，肺泡巨噬细胞与DC具有一些共同的物理特性，这使得制备纯净的肺APC群体变得复杂。为了克服这些困难，我们开发了改进的基于流式细胞术的方法，用于分析和纯化来自肺及其引流淋巴结（LN）的APC。在本章中，我们将详细描述这些方法，以及培养APC并表征其与T细胞相互作用的方法。
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本文引用的文献
1
Pulmonary CD103(+) dendritic cells prime Th2 responses to inhaled allergens.肺脏 CD103(+)树突状细胞对吸入性变应原引发 Th2 反应。
Mucosal Immunol. 2012 Jan;5(1):53-65. doi: 10.1038/mi.2011.47. Epub 2011 Oct 19.
2
Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209(+) dendritic cells for immune T cell areas.微生物刺激可使单核细胞完全分化为表达 DC-SIGN/CD209(+)的树突状细胞，用于免疫 T 细胞区。
Cell. 2010 Oct 29;143(3):416-29. doi: 10.1016/j.cell.2010.09.039.
3
Methods to study pulmonary dendritic cell migration.研究肺树突状细胞迁移的方法。
Methods Mol Biol. 2010;595:371-82. doi: 10.1007/978-1-60761-421-0_24.
4
Biology of lung dendritic cells at the origin of asthma.哮喘起源中肺树突状细胞的生物学特性
Immunity. 2009 Sep 18;31(3):412-24. doi: 10.1016/j.immuni.2009.08.008.
5
Blood-derived inflammatory dendritic cells in lymph nodes stimulate acute T helper type 1 immune responses.淋巴结中源自血液的炎性树突状细胞刺激急性1型辅助性T细胞免疫反应。
Nat Immunol. 2009 Apr;10(4):394-402. doi: 10.1038/ni.1707. Epub 2009 Mar 1.
6
Identification of clonogenic common Flt3+M-CSFR+ plasmacytoid and conventional dendritic cell progenitors in mouse bone marrow.小鼠骨髓中克隆形成性共同Flt3+M-CSFR+浆细胞样和传统树突状细胞祖细胞的鉴定。
Nat Immunol. 2007 Nov;8(11):1207-16. doi: 10.1038/ni1518. Epub 2007 Oct 7.
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Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo.体外和体内来源的单个前体细胞向浆细胞样树突状细胞和传统树突状细胞亚型的发育。
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9
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