Nakano Hideki, Cook Donald N
Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
Methods Mol Biol. 2013;1032:19-29. doi: 10.1007/978-1-62703-496-8_2.
Antigen presenting cells (APCs) such as dendritic cells (DCs) and macrophages comprise a relatively small fraction of leukocytes residing in lymphoid and non-lymphoid tissues. Accordingly, functional analyses of these cells have been hampered by low cell yields. Also, alveolar macrophages share several physical properties with DCs, and this has complicated efforts to prepare pure populations of lung APCs. To overcome these difficulties, we have developed improved flow cytometry-based methods to analyze and purify APCs from the lung and its draining lymph nodes (LNs). In this chapter, we describe these methods in detail, as well as methods for culturing APCs and characterizing their interactions with T cells.
抗原呈递细胞（APC），如树突状细胞（DC）和巨噬细胞，在驻留在淋巴组织和非淋巴组织中的白细胞中所占比例相对较小。因此，这些细胞的功能分析因细胞产量低而受到阻碍。此外，肺泡巨噬细胞与DC具有一些共同的物理特性，这使得制备纯净的肺APC群体变得复杂。为了克服这些困难，我们开发了改进的基于流式细胞术的方法，用于分析和纯化来自肺及其引流淋巴结（LN）的APC。在本章中，我们将详细描述这些方法，以及培养APC并表征其与T细胞相互作用的方法。
