Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Linnanmaa, Oulu, Finland.
Proteomics. 2010 Feb;10(3):555-63. doi: 10.1002/pmic.200800657.
2-DE reference maps for Deinococcus geothermalis cytosolic and cell envelope proteomes were constructed. In total, 403 spots were identified as 299 different proteins. Unique in the proteomes were four subunits of V-type ATPase and Deinococcus specific proteins constituting one-fourth of cell envelope proteome. The cytoplasmic proteome included enzymes of the central carbon metabolism, chaperones, enzymes of protein and DNA repair, and oxidative stress. A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis.
构建了地热能异常球菌胞质和细胞包膜蛋白质组的 2-DE 参考图谱。共鉴定出 403 个斑点，分别为 299 种不同的蛋白质。蛋白质组中独特的是 V 型 ATP 酶的四个亚基和构成细胞包膜蛋白质组四分之一的 Deinococcus 特异性蛋白质。细胞质蛋白质组包括中心碳代谢的酶、伴侣蛋白、蛋白质和 DNA 修复酶以及氧化应激酶。总共 34 种丰富的具有未知功能的蛋白质可能与地热能异常球菌的极端应激耐受性有关。
