Tian Bing, Wang Hu, Ma Xiaoqiong, Hu Yaping, Sun Zongtao, Shen Shaochuan, Wang Fei, Hua Yuejin
Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, 310029 Hangzhou, China.
Mol Biosyst. 2010 Oct;6(10):2068-77. doi: 10.1039/c004875e. Epub 2010 Aug 11.
Deinococcus geothermalis is a radioresistant and moderate thermophilic bacterium. Little was known about the membrane or membrane associated proteins of this bacterium. This study established the membrane subproteome profile of D. geothermalis, using 1-D PAGE and LC-MS/MS analysis following Triton X-114 detergent extraction. A total of 552 proteins from the membrane preparations were identified from two independent trials. In the total identified proteins, 117 were membrane subproteomic proteins, and 89 of them were described for the first time in D. geothermalis including fimbrial pilin (Dgeo_2038), cytochrome bd ubiquinol oxidase (Dgeo_2705) and multi-sensor (Dgeo_2096). The major membrane subproteomic proteins were distributed into 18 functional groups including nutrient transport and metabolism, energy production and conversion, cell wall/membrane biogenesis and a poorly characterized subclass. The identifications of Deinococcus-specific proteins, such as cell surface receptor IPT/TIG (Dgeo_1119) and four hypothetical proteins, demonstrated the special protein composition and functions in the cell membrane of Deinococcus. The results provide a basis for quantitative proteomic analysis, which will facilitate the understanding of the adaptation of this organism to different environmental stresses and the development of strategies for bioremediation of environmental waste.
嗜热栖热放线菌是一种耐辐射且嗜温适中的细菌。关于这种细菌的膜或膜相关蛋白，人们了解甚少。本研究通过Triton X - 114去污剂提取后进行一维聚丙烯酰胺凝胶电泳（1 - D PAGE）和液相色谱 - 串联质谱（LC - MS/MS）分析，建立了嗜热栖热放线菌的膜亚蛋白质组图谱。在两次独立试验中，共鉴定出552种来自膜制剂的蛋白质。在总共鉴定出的蛋白质中，有117种是膜亚蛋白质组蛋白，其中89种是在嗜热栖热放线菌中首次被描述的，包括菌毛菌毛蛋白（Dgeo_2038）、细胞色素bd泛醇氧化酶（Dgeo_2705）和多传感器（Dgeo_2096）。主要的膜亚蛋白质组蛋白分布在18个功能组中，包括营养物质运输与代谢、能量产生与转换、细胞壁/膜生物合成以及一个特征不明的亚类。嗜热栖热放线菌特异性蛋白的鉴定，如细胞表面受体IPT/TIG（Dgeo_1119）和四种假定蛋白，证明了嗜热栖热放线菌细胞膜中特殊的蛋白质组成和功能。这些结果为定量蛋白质组分析提供了基础，这将有助于理解这种生物体对不同环境压力的适应性以及开发环境废物生物修复策略。
