State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
Dalton Trans. 2011 Dec 28;40(48):12818-25. doi: 10.1039/c1dt10996k. Epub 2011 Aug 30.
Rattle-type hollow nanocapsules are among of the most promising candidates as drug carriers owing to their huge inner space and multifunctional material combination. In this paper, rattle-type hollow CaWO(4):Tb(3+)@SiO(2) nanocapsules with a diameter of 100-110 nm and a wall thickness around 10 nm were fabricated. The hollow silica nanospheres were used as nano-reactors and the luminescent core of CaWO(4):Tb(3+) was post-filled into the nano-reactors by a vacuum nano-casting route combined with a Pechini-type sol-gel method. Subsequently, doxorubicin hydrochloride (DOX), a model of an anti-cancer drug, is loaded into the CaWO(4):Tb(3+)@SiO(2) nanocapsules and their cell cytotoxicity, cancer cell uptake and drug release behavior are investigated in vitro. The prepared multifunctional inorganic nanocapsules show a loading capacity for DOX as high as 124 mg g(-1) and sustained-release properties. The release profile of the drug from DOX-loaded nanocapsules can last over five days. Besides, the blank CaWO(4):Tb(3+)@SiO(2) shows very low cytotoxicity against cancer cell lines (HeLa cell) while the DOX-loaded nanocapsules exhibit relatively high efficiency for killing of HeLa cells. The rapid cancer cell uptake process is observed by confocal laser scanning microscopy. The results indicate that a rattle-type hollow CaWO(4):Tb(3+)@SiO(2) nanocapsule has the potential to be used as drug carrier in therapy. Moreover, it is possible to extend the synthetic strategy in this study to other rattle-type multifunctional composites to meet various demands.
摇铃型中空纳米胶囊因其具有巨大的内部空间和多功能材料组合，成为最有前途的药物载体之一。本文制备了一种直径为 100-110nm、壁厚约 10nm 的摇铃型中空 CaWO(4):Tb(3+)@SiO(2)纳米胶囊。中空硅纳米球被用作纳米反应器，通过真空纳米铸造法与 Pechini 型溶胶-凝胶法相结合，将发光核 CaWO(4):Tb(3+)后填充到纳米反应器中。随后，将盐酸阿霉素（DOX）作为抗癌药物的模型载入到 CaWO(4):Tb(3+)@SiO(2)纳米胶囊中，并对其体外细胞毒性、癌细胞摄取和药物释放行为进行了研究。所制备的多功能无机纳米胶囊对 DOX 的载药能力高达 124mg g(-1)，具有持续释放性能。药物从载药纳米胶囊中的释放曲线可持续五天以上。此外，空白 CaWO(4):Tb(3+)@SiO(2)对癌细胞系（HeLa 细胞）的细胞毒性非常低，而载药纳米胶囊对 HeLa 细胞的杀伤效率相对较高。通过共聚焦激光扫描显微镜观察到癌细胞的快速摄取过程。结果表明，摇铃型中空 CaWO(4):Tb(3+)@SiO(2)纳米胶囊有望作为治疗中的药物载体。此外，本研究中的合成策略有可能扩展到其他摇铃型多功能复合材料，以满足各种需求。
