CIIMAR/CIMAR-LA, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal.
Res Microbiol. 2010 Jan-Feb;161(1):9-17. doi: 10.1016/j.resmic.2009.11.001. Epub 2009 Nov 26.
A polyphasic study of a benthic Nodularia isolate (LEGE06071) from an Atlantic environment, specifically salt pans, was performed. LEGE06071 resembled both type strains of Nodularia sphaerocarpa and Nodularia harveyana, while ACOI00729 (purchased isolate) was identified as N. sphaerocarpa. The length and width of vegetative cells varied from 3.10 to 3.15 microm and from 3.71 to 4.25 microm, respectively, while heterocyts were 3.91-4.89 microm long and 4.20-4.74 microm wide. None of the isolates had aerotopes. The sequencing of the 16S rRNA gene from the two Nodularia isolates indicated that they belonged neither to Nodularia spumigena nor N. harveyana. Nodularin and other cyanotoxin synthesis-associated genes could not be detected, nor could nodularin production be detected by ELISA. However, MALDI-TOF analysis of LEGE06071 revealed the presence of other compounds, namely, glycolipids. Hence, toxicological screenings against Artemia nauplii, Escherichia coli and Salmonella typhimurium were performed. Toxic effects could only be observed against Artemia, with 48 h-LC(50) values for the aqueous and crude extract of methanol of 53.21 mg ml(-1) and 17.81 mg ml(-1), respectively. This study presents the first evidence of a non-nodularin-producing Nodularia isolate in Atlantic salt pan ecosystems and its potential ecotoxicity against Artemia sp.
对一种来自大西洋环境（具体为盐田）的底栖浮霉状蓝藻（LEGE06071）进行了多相研究。LEGE06071 与球形浮霉状蓝藻和 Harveyana nodularia 的两种模式株均相似，而 ACOI00729（购买的分离株）则被鉴定为球形浮霉状蓝藻。营养细胞的长度和宽度分别为 3.10-3.15 微米和 3.71-4.25 微米，异形胞长 3.91-4.89 微米，宽 4.20-4.74 微米。没有分离株具有气腔。对两个浮霉状蓝藻分离株的 16S rRNA 基因测序表明，它们既不属于浮霉状鱼腥藻也不属于 Harveyana nodularia。未检测到鱼腥藻毒素和其他与蓝藻毒素合成相关的基因，也无法通过 ELISA 检测到鱼腥藻毒素的产生。然而，LEGE06071 的 MALDI-TOF 分析显示存在其他化合物，即糖脂。因此，对卤虫、大肠杆菌和鼠伤寒沙门氏菌进行了毒理学筛选。仅对卤虫观察到毒性作用，其水相和甲醇粗提物的 48 h-LC(50)值分别为 53.21 mg ml(-1)和 17.81 mg ml(-1)。本研究首次证明了大西洋盐田生态系统中存在一种不产鱼腥藻毒素的浮霉状蓝藻分离株及其对卤虫的潜在生态毒性。
