CESAM & Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Environ Pollut. 2010 May;158(5):1783-90. doi: 10.1016/j.envpol.2009.11.007. Epub 2009 Nov 26.
The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a downregulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses.
采用传统和新型生物标志物相结合的方法，评估了斜带石斑鱼和欧洲鲈鱼对受污染沿海泻湖的水生污染的反应。彗星试验评估的 DNA 损伤在受污染地点的两种鱼类中均较高，而细胞色素 P450 1A1 基因表达水平没有明显改变。肝组织病理学分析也显示出受污染地点鱼类的明显病变。分析了 ras 和 xpf 基因的改变，并鉴定了其他对污染物有反应的基因。ras 基因没有发现改变，而在受污染地点的 D. labrax 中，xpf 基因的表达下调。应用于受污染地点采集的 D. labrax 的抑制性消减杂交显示，参与能量代谢、免疫系统活性和抗氧化反应的基因表达发生改变。本文报道的方法和结果证明了将传统生物标志物反应与新型生物标志物反应相结合的有效性。
