Laboratory of Microbiology (WE 10), Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, KL Ledeganckstraat 35, 9000 Ghent, Belgium.
Syst Appl Microbiol. 2010 Jan;33(1):7-14. doi: 10.1016/j.syapm.2009.10.001. Epub 2009 Nov 28.
Arcobacter cryaerophilus is a heterogeneous species in which two distinct subgroups have been reported. In the present study, the taxonomic status of these subgroups was reassessed using amplified fragment length polymorphism and heat shock protein 60 gene sequence analysis. The results demonstrated that A. cryaerophilus has a complex taxonomic structure, which consists of multiple cores of strains that share intermediate levels of DNA-DNA hybridisation and exhibit low levels of DNA-DNA hybridisation towards other Arcobacter species. One of these cores consisted of the majority of strains and included most subgroup 2 strains from previous studies. A. cryaerophilus subgroup 1 strains represented three distinct cores, among which the type strain occupied a distinct position. These results therefore also demonstrate that the current subgroup nomenclature in A. cryaerophilus should be abandoned, and that the type strain is genetically aberrant and poorly represents strains belonging to this species.
嗜冷弯曲菌是一个具有高度异质性的物种，其中已经报道了两个不同的亚群。在本研究中，使用扩增片段长度多态性和热休克蛋白 60 基因序列分析重新评估了这些亚群的分类地位。结果表明，嗜冷弯曲菌具有复杂的分类结构，由多个具有中间 DNA-DNA 杂交水平的菌株核心组成，并且与其他弯曲菌属物种的 DNA-DNA 杂交水平较低。其中一个核心由大多数菌株组成，包括之前研究中的大多数亚群 2 菌株。嗜冷弯曲菌亚群 1 菌株代表三个不同的核心，其中包括模式株。因此，这些结果还表明，嗜冷弯曲菌中当前的亚群命名法应该被废弃，而模式株在遗传上是异常的，并且不能很好地代表属于该物种的菌株。
