Collado Luis, Cleenwerck Ilse, Van Trappen Stefanie, De Vos Paul, Figueras Maria Jose
Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Sant Llorenç 21, 43201 Reus, Spain.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1391-6. doi: 10.1099/ijs.0.003749-0.
Three Arcobacter isolates, recovered from mussels (genus Mytilus), and one isolate from brackish water in Catalonia (north-east Spain) showed a novel pattern using a recently described identification method for members of the genus Arcobacter, 16S rRNA gene RFLP. Enterobacterial repetitive intergenic consensus PCR fingerprinting demonstrated that the three isolates from mussels belonged to two genotypes and that the fourth isolate from water belonged to a third genotype. Analysis of the 16S rRNA and rpoB gene sequences showed that the new isolates formed a separate lineage within the genus Arcobacter. This was also confirmed by the low DNA-DNA relatedness values (16-30 %) of the isolates with the type strains of recognized Arcobacter species. Hydrolysis of indoxyl acetate, a characteristic trait for all species of the genus Arcobacter, was negative for the novel isolates. The susceptibility of the novel isolates to cefoperazone, together with the lack of urease production and nitrate reduction, further enabled them to be differentiated from recognized Arcobacter species based on physiological characteristics. Genotypic and phenotypic characteristics indicated that the new isolates represent a novel species of the genus Arcobacter, for which the name Arcobacter mytili sp. nov. is proposed, with the type strain F2075(T) (=CECT 7386(T) =LMG 24559(T)). The DNA G+C content of strain F2075(T) was 26.9 mol%.
从贻贝（紫贻贝属）中分离出的三株弓形杆菌，以及从西班牙东北部加泰罗尼亚的微咸水中分离出的一株弓形杆菌，采用最近描述的用于弓形杆菌属成员鉴定的方法——16S rRNA基因RFLP，呈现出一种新的模式。肠杆菌重复基因间共有序列PCR指纹图谱显示，来自贻贝的三株分离菌属于两种基因型，而来自水中的第四株分离菌属于第三种基因型。16S rRNA和rpoB基因序列分析表明，新分离菌在弓形杆菌属内形成了一个独立的谱系。分离菌与已确认的弓形杆菌属模式菌株的低DNA-DNA相关性值（16-30%）也证实了这一点。吲哚乙酸水解是弓形杆菌属所有物种的一个特征性性状，新分离菌对此呈阴性。新分离菌对头孢哌酮的敏感性，以及缺乏脲酶产生和硝酸盐还原能力，进一步使其能够根据生理特征与已确认的弓形杆菌属物种区分开来。基因型和表型特征表明，新分离菌代表了弓形杆菌属的一个新物种，为此提出名称为Arcobacter mytili sp. nov.，模式菌株为F2075(T)（=CECT 7386(T)=LMG 24559(T)）。菌株F2075(T)的DNA G+C含量为26.9 mol%。
