Houf Kurt, On Stephen L W, Coenye Tom, Debruyne Lies, De Smet Sarah, Vandamme Peter
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2599-604. doi: 10.1099/ijs.0.006650-0. Epub 2009 Jul 21.
During a Danish study on the prevalence of campylobacteria in pig abortions and food of animal origin, eight Gram-negative, slightly curved, rod-shaped, non-spore-forming bacteria were clustered by using amplified fragment length polymorphism analysis in a distinct phenon within the genus Arcobacter. In the present study, numerical analysis of whole-cell protein profiles also showed that all isolates clustered in a single group distinct from other recognized Arcobacter species. DNA-DNA hybridization among two representative strains exhibited a mean DNA-DNA relatedness value of 79 %. DNA-DNA hybridization with the type strains of recognized Arcobacter species revealed levels of DNA-DNA relatedness of 41 % or less. The DNA G+C content of the type strain was 28.5 mol%. Pairwise comparison of the 16S rRNA gene sequences with those of the type strains of established species identified Arcobacter cryaerophilus (97.9 %), Arcobacter cibarius (97.5 %) and Arcobacter skirrowii (97.2 %) as the nearest phylogenetic neighbours. The isolates could be distinguished from other Arcobacter species by means of the following biochemical tests: activities of catalase and urease, reduction of nitrate and growth on minimal medium, lack of growth at 37 degrees C under standardized aerobic and microaerobic conditions, in 4 % NaCl and 1 % glycine media. Finally, DNA fingerprints obtained by using enterobacterial repetitive intergenic consenus-PCR showed that the eight isolates represent eight strains of a single novel Arcobacter species, for which the name Arcobacter thereius sp. nov. is proposed. The type strain is LMG 24486(T) (=CCUG 56902(T)).
在丹麦一项关于猪流产及动物源性食品中弯曲杆菌属患病率的研究中，利用扩增片段长度多态性分析，8株革兰氏阴性、稍弯曲、杆状、不形成芽孢的细菌在嗜冷栖热菌属内聚为一个独特的表型群。在本研究中，全细胞蛋白质谱的数值分析也表明，所有分离株聚为一个与其他已确认的嗜冷栖热菌属物种不同的单一组群。两株代表性菌株之间的DNA-DNA杂交显示平均DNA-DNA相关性值为79%。与已确认的嗜冷栖热菌属物种的模式菌株进行DNA-DNA杂交，结果显示DNA-DNA相关性水平为41%或更低。模式菌株的DNA G+C含量为28.5 mol%。将16S rRNA基因序列与已确立物种的模式菌株进行成对比较，确定嗜冷栖热弯曲杆菌（97.9%）、食源性弯曲杆菌（97.5%）和斯氏弯曲杆菌（97.2%）为最近的系统发育邻种。这些分离株可通过以下生化试验与其他嗜冷栖热菌属物种区分开来：过氧化氢酶和脲酶活性、硝酸盐还原以及在基本培养基上的生长情况，在标准化需氧和微需氧条件下37℃不生长，在4%氯化钠和1%甘氨酸培养基中不生长。最后，利用肠杆菌重复基因间共有序列PCR获得的DNA指纹图谱表明，这8株分离株代表一个单一新嗜冷栖热菌属物种的8个菌株，为此提出新物种名称嗜冷栖热栖居嗜冷栖热菌。模式菌株为LMG 24486(T)（=CCUG 56902(T)）。
