Microbial Technology for Marine Pollution Treatment Research Unit, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Environ Sci Pollut Res Int. 2018 Sep;25(27):26927-26938. doi: 10.1007/s11356-018-2742-3. Epub 2018 Jul 14.
Serratia sp. W4-01 was immobilized in chitosan-activated carbon beads and used for diesel oil removal. The type and concentration of chitosan, activated carbon content, and bead diameter were investigated as factors affecting diesel oil removal. The results showed that 2% (w/v) squid pen chitosan beads modified with 1% activated carbon (w/v) and with a 3-mm diameter had a good spherical shape and strength as well as diesel oil removal capability. The immobilized W4-01 cells removed more than 40% of diesel oil after 7 days when the initial diesel oil concentration was 100 to 400 mg L, whereas 29-36% of diesel oil was removed after 14 days when the initial concentration was 800 to 1000 mg L. Additionally, the immobilized cells maintained the ability to remove diesel oil over a pH range of 5-11. The addition of a biosurfactant increased the diesel oil removal from 62 to 75%. The reusability tests revealed that the ability of immobilized cells to remove diesel oil was enhanced after reuse, and 50-90% of diesel oil was removed during 2 to 12 reuse cycles. The stability and survival of W4-01 cells was confirmed by scanning electron microscopy and confocal laser scanning microscopy. The results of this study showed the potential use of W4-01 cells immobilized in chitosan-activated carbon beads for future applications in remediating diesel contamination.
希瓦氏菌 W4-01 被固定在壳聚糖活性炭珠中，用于去除柴油。考察了壳聚糖的类型和浓度、活性炭含量和珠径等因素对柴油去除的影响。结果表明，2%（w/v）鱿鱼墨壳聚糖珠用 1%（w/v）活性炭改性，粒径为 3mm，具有良好的球形和强度，以及去除柴油的能力。当初始柴油浓度为 100-400mg/L 时，固定化 W4-01 细胞在 7 天后去除了超过 40%的柴油，而当初始浓度为 800-1000mg/L 时，14 天后去除了 29-36%的柴油。此外，固定化细胞在 pH 值为 5-11 的范围内保持去除柴油的能力。添加生物表面活性剂可将柴油去除率从 62%提高到 75%。重复使用试验表明，固定化细胞去除柴油的能力在重复使用后得到增强，在 2-12 次重复使用循环中可去除 50-90%的柴油。扫描电子显微镜和共聚焦激光扫描显微镜证实了 W4-01 细胞的稳定性和存活能力。这项研究的结果表明，用壳聚糖活性炭珠固定化的 W4-01 细胞在未来修复柴油污染方面具有潜在的应用价值。
