唐氏综合征细胞粘附分子（DSCAM）和唐氏综合征细胞粘附分子样蛋白1（DSCAML1）在发育中的小鼠视网膜的多种细胞类型的自我回避中发挥作用。
DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina.
作者信息
Fuerst Peter G, Bruce Freyja, Tian Miao, Wei Wei, Elstrott Justin, Feller Marla B, Erskine Lynda, Singer Joshua H, Burgess Robert W
机构信息
The Jackson Laboratory, Bar Harbor, ME 04609, USA.
出版信息
Neuron. 2009 Nov 25;64(4):484-97. doi: 10.1016/j.neuron.2009.09.027.
DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In addition to a subset of amacrine cells, Dscam was expressed in most retinal ganglion cells (RGCs). RGCs had fasciculated dendrites and clumped cell bodies in Dscam(-/-) mice, suggesting a role in self-avoidance. Dscaml1 was expressed in the rod circuit, and mice lacking Dscaml1 had fasciculated rod bipolar cell dendrites and clumped AII amacrine cell bodies, also indicating a role in self-avoidance. Neurons in Dscam or Dscaml1 mutant retinas stratified their processes appropriately in synaptic laminae in the inner plexiform layer, and functional synapses formed in the rod circuit in mice lacking Dscaml1. Therefore, DSCAM and DSCAML1 function similarly in self-avoidance, and are not essential for synaptic specificity in the mouse retina.
取决于所研究的物种、细胞类型和基因家族成员，唐氏综合征细胞粘附分子（DSCAM）和类唐氏综合征细胞粘附分子1（DSCAML1）发挥多种神经发育功能，包括轴突导向、突触粘附和自我回避。我们研究了DSCAM和DSCAML1在发育中的小鼠视网膜中的功能。除了一部分无长突细胞外，Dscam在大多数视网膜神经节细胞（RGC）中表达。在Dscam基因敲除小鼠中，RGC的树突呈束状，细胞体聚集，这表明其在自我回避中发挥作用。Dscaml1在视杆细胞通路中表达，缺乏Dscaml1的小鼠视杆双极细胞的树突呈束状，AII无长突细胞体聚集，这也表明其在自我回避中发挥作用。Dscam或Dscaml1突变视网膜中的神经元在视网膜内丛状层的突触层中其突起分层适当，并且在缺乏Dscaml1的小鼠视杆细胞通路中形成了功能性突触。因此，DSCAM和DSCAML1在自我回避中的功能相似，对小鼠视网膜中的突触特异性并非必不可少。
Zotero 插件