含膜噬菌体PRD1及其亚病毒颗粒的大规模纯化。
Large-scale purification of membrane-containing bacteriophage PRD1 and its subviral particles.
作者信息
Bamford J K, Bamford D H
机构信息
Department of Genetics, University of Helsinki, Finland.
出版信息
Virology. 1991 Mar;181(1):348-52. doi: 10.1016/0042-6822(91)90501-2.
PMID:1994582
Abstract
PRD1 is a dsDNA virus that infects Escherichia coli and Salmonella typhimurium. The genome is a linear molecule with 5' covalently linked terminal protein. The virus has a lipid membrane inside the protein coat. We describe the large-scale purification of the virus using a zonal rotor and the yields and quality of the virus and its subviral assemblies for subsequent biophysical measurements.
摘要
PRD1是一种双链DNA病毒，可感染大肠杆菌和鼠伤寒沙门氏菌。其基因组是一个线性分子，带有5' 共价连接的末端蛋白。该病毒在蛋白衣壳内部有一层脂质膜。我们描述了使用区带转头对该病毒进行大规模纯化，以及病毒及其亚病毒组件的产量和质量，以便后续进行生物物理测量。
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