Raheb Jamshid, Hajipour Mohammad Javad, Saadati Mojtaba, Rasekh Behnam, Memari Babak
National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.Box.14155-6343, Tehran, Iran.
Iran Biomed J. 2009 Oct;13(4):207-13.
The combustion of sulfur-rich fossil fuels leads to release of sulfur oxide pollution in the environment. In biodesulfurization process, an organism is able to remove sulfur from fossil fuels without decreasing the caloric value of those substrates. The main aim of this research was to design a recombinant microorganism to remove the highest amount of sulfur compounds in fossil fuels.
Three genes (dszA,B,C) from dsz operon are responsible for the 4S pathway (biodesulfurization pathway) in Rhodococcus erythropolis IGTS8 were inserted into the chromosome of a novel indigenous Pseudomonas putida. The reaction catalyzed by products of dszA,B,C genes require FMNH2 supplied by dszD enzyme. Thus, pVLT31 vector harboring dszD gene was transferred into this recombinant strain.
The results demonstrated a higher biodesulfurization activity when the flavin reductase gene was transferred into recombinant P. putida harboring dszA,B,C. These results were approved by the Gibbs test and HPLC analysis.
These analyses showed that this novel indigenous engineered P. putida could be a promising candidate for an industrial and environmental application for Biodesulfurization process.
富含硫的化石燃料燃烧会导致环境中硫氧化物污染的释放。在生物脱硫过程中，一种生物体能够从化石燃料中去除硫而不降低这些底物的热值。本研究的主要目的是设计一种重组微生物，以去除化石燃料中含量最高的硫化合物。
来自红平红球菌IGTS8中dsz操纵子的三个基因（dszA、B、C）负责4S途径（生物脱硫途径），将其插入到一种新的本地恶臭假单胞菌的染色体中。dszA、B、C基因产物催化的反应需要由dszD酶提供的FMNH2。因此，携带dszD基因的pVLT31载体被转入该重组菌株。
当黄素还原酶基因转入携带dszA、B、C的重组恶臭假单胞菌时，结果显示出更高的生物脱硫活性。这些结果通过吉布斯试验和高效液相色谱分析得到了证实。
这些分析表明，这种新型的本地工程化恶臭假单胞菌可能是生物脱硫过程在工业和环境应用中的一个有前途的候选者。
