Salih K M, Jusuf N H, Hamid A A, Yusoff W M W
Department of Microbiology, Faculty of Veterinary Science, University of Bahar E1-Gahzal, 10739, Khartoum, Sudan.
Pak J Biol Sci. 2009 Jul 15;12(14):1036-40. doi: 10.3923/pjbs.2009.1036.1040.
In the present study, Ten soil samples were examined and the pH of the soil was recorded. For bacterial isolation, a sterile nutrient and blood agars were used. Gram stain and biochemical tests were done for identification. A total of 384 genus were isolated, 314 (81.8%) were identified as Pseudomonas species of which 245 (78.0%) were Pseudomonas aeruginosa, 42 (13.4%) were Pseudomonas fluorescens, 13 (4.2%) were Pseudomonas mallei, 10 (3.1%) were Pseudomonas putida and 4 (1.3%) were Pseudomonas syringe and are regarded as pathogenic and harmful to man, animal and plants. This study shows that Pseudomonas aeruginosa had a high adaptation capability to grow in soil samples from Ternate, Indonesia. The rest of the bacterial isolates (18.2%) were identified as follows: 24 samples (6.2%) were Micrococcus, 23 samples (6.0%) were E. coli, 12 samples (3.1%) were Pasteurella and 11 samples (2.9%) were Staphylococcus. Pencillium was also isolated.
在本研究中，对十个土壤样本进行了检测并记录了土壤的pH值。为了进行细菌分离，使用了无菌营养琼脂和血琼脂。通过革兰氏染色和生化试验进行鉴定。总共分离出384个属，其中314个（81.8%）被鉴定为假单胞菌属，其中245个（78.0%）是铜绿假单胞菌，42个（13.4%）是荧光假单胞菌，13个（4.2%）是鼻疽假单胞菌，10个（3.1%）是恶臭假单胞菌，4个（1.3%）是丁香假单胞菌，它们被认为对人类、动物和植物具有致病性和危害性。本研究表明，铜绿假单胞菌在印度尼西亚特尔纳特的土壤样本中具有很高的生长适应能力。其余的细菌分离株（18.2%）鉴定如下：24个样本（6.2%）是微球菌，23个样本（6.0%）是大肠杆菌，12个样本（3.1%）是巴斯德氏菌，11个样本（2.9%）是葡萄球菌。还分离出了青霉菌。
