Shandong Peanut Research Institute, Wannianquan Road 126, Qingdao, 266100, China.
Arch Microbiol. 2019 Jul;201(5):673-678. doi: 10.1007/s00203-019-01636-w. Epub 2019 Feb 23.
A Gram-stain-negative, aerobic, mobile, and rod-shaped bacterium, designated JJ3, was isolated from peanut rhizospheric soil in Qingdao, Shandong Province, China, and was characterized using a polyphasic approach. Strain JJ3 grew at 4-40 °C, at pH 5.0-9.0 and 0-4% NaCl. The strain was positive for both catalase and oxidase tests, and was able to degrade aflatoxin B. According to the 16S rRNA gene sequence comparisons, the strain JJ3 was identified as a member of the genus Pseudomonas and was most closely related to Pseudomonas japonica JCM 21532 and Pseudomonas alkylphenolica JCM 16553 with sequence similarity of 99.0% and 98.9%, respectively. A multilocus sequence analysis (MLSA) of concatenating 16S rRNA, gyrB and rpoD gene sequences showed that strain JJ3 belonged to the Pseudomonas putida subcluster. Genomic comparison of strain JJ3 with its closest phylogenetic type strain using average nucleotide index (ANI) and digital DNA-DNA relatedness revealed 76.7-82.9% and 20.2-37.1%, respectively. All values were distinctly lower than the thresholds established for species differentiation. The predominant cellular fatty acids of strain JJ3 were C17:0 cyclo (24.0%), C (21.4%), summed features 3 (Cω7c and/or Cω6c) (11.5%) and summed features 8 (Cω7c and/or Cω6c) (10.5%). The major polar lipids of strain JJ3 were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The physiological, biochemical, and genetic characteristics support the assignment of JJ3 to the genus Pseudomonas, but are different to those of phylogenetically neighboring species to represent a novel species. The name Pseudomonas qingdaonensis sp. nov. is proposed, with JJ3 (= JCM 32579 = KCTC 62384 = CGMCC 1.16493) as the type strain.
一株革兰氏阴性、需氧、运动和杆状细菌，被命名为 JJ3，从中国山东省青岛市花生根际土壤中分离得到，并通过多相研究进行了描述。菌株 JJ3 在 4-40°C、pH5.0-9.0 和 0-4%NaCl 条件下生长。该菌株过氧化氢酶和氧化酶试验均为阳性，能够降解黄曲霉毒素 B。根据 16S rRNA 基因序列比较，菌株 JJ3 被鉴定为假单胞菌属的成员，与 Pseudomonas japonica JCM 21532 和 Pseudomonas alkylphenolica JCM 16553 的相似度最高，序列相似性分别为 99.0%和 98.9%。对 16S rRNA、gyrB 和 rpoD 基因序列的多位点序列分析（MLSA）表明，菌株 JJ3 属于 Pseudomonas putida 亚群。用平均核苷酸指数（ANI）和数字 DNA-DNA 相关性比较菌株 JJ3 与其最接近的系统发育型菌株的基因组，结果分别为 76.7-82.9%和 20.2-37.1%。所有数值均明显低于物种分化的阈值。菌株 JJ3 的主要细胞脂肪酸为 C17:0 环（24.0%）、C（21.4%）、总和特征 3（Cω7c 和/或 Cω6c）（11.5%）和总和特征 8（Cω7c 和/或 Cω6c）（10.5%）。菌株 JJ3 的主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。生理、生化和遗传特征支持将 JJ3 分配到假单胞菌属，但与系统发育上相邻的种不同，代表一个新种。提议将 JJ3 命名为青岛假单胞菌 sp. nov.，其 JJ3（=JCM 32579=KCTC 62384=CGMCC 1.16493）为模式株。
