University of Pennsylvania, Department of Pathology and Laboratory Medicine, 613A Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA.
J Proteome Res. 2010 Feb 5;9(2):700-7. doi: 10.1021/pr900256v.
"Multi-stage" search strategies have become widely accepted for peptide identification and are implemented in a number of available software packages. We describe limitations of these strategies for validation and decoy-based statistical analyses and demonstrate these limitations using a set of control sample spectra. We propose a solution that corrects the statistical deficiencies and describe its implementation using the open-source software X!Tandem.
"多阶段"搜索策略已被广泛接受用于肽鉴定，并在许多可用的软件包中实现。我们描述了这些策略在验证和基于诱饵的统计分析方面的局限性，并使用一组对照样品谱来证明这些局限性。我们提出了一种解决方案来纠正统计缺陷，并描述了使用开源软件 X!Tandem 来实现该方案。
