Faculty of Technology, University of Novi Sad, 21000 Novi Sad, Serbia.
Bioresour Technol. 2010 Apr;101(7):2167-72. doi: 10.1016/j.biortech.2009.11.020. Epub 2009 Nov 30.
The ability of coagulation active proteins from common bean (Phaseolus vulgaris) seed for the removal of water turbidity was studied. Partial purification of protein coagulant was performed by precipitation with ammonium sulphate, dialysis and anion exchange chromatography. Adsorption parameters for ion-exchange process were established using dialysate extract. Results revealed that the highest values of the adsorbed protein were achieved in 50 mmol/L phosphate buffer at pH 7.5 and the maximum adsorption capacity was calculated to be 0.51 mg protein/mL matrix. Partially purified coagulant at initial turbidity 35 NTU expressed the highest value of coagulation activity, 72.3%, which was almost 22 times higher than those obtained by crude extract considering applied dosages. At the same time, the increase in organic matter that remained in water after coagulation with purified protein coagulant was more than 16 times lower than those with crude extract, relatively to its content in blank.
研究了普通菜豆（Phaseolus vulgaris）种子中凝血活性蛋白去除水浊度的能力。通过硫酸铵沉淀、透析和阴离子交换层析对蛋白凝固剂进行了部分纯化。使用透析液提取物确定了离子交换过程的吸附参数。结果表明，在 pH 值为 7.5 的 50mmol/L 磷酸盐缓冲液中，吸附蛋白的含量最高，最大吸附容量计算为 0.51mg 蛋白/mL 基质。在初始浊度为 35NTU 的情况下，部分纯化的凝固剂表现出最高的凝固活性，为 72.3%，考虑到应用剂量，几乎比粗提物高 22 倍。同时，与空白相比，用纯化的蛋白凝固剂凝固后残留在水中的有机物增加了 16 倍以上，相对于其在空白中的含量。
