Cell and Developmental Biology Department, Institute of Biomedical Sciences I, University of São Paulo, São Paulo, Brazil.
Cytokine. 2010 Jan;49(1):102-8. doi: 10.1016/j.cyto.2009.10.007. Epub 2009 Nov 30.
Heart failure (HF) is associated with changes in the skeletal muscle (SM) which might be a consequence of the unbalanced local expression of pro- (TNF-alpha) and anti- (IL-10) inflammatory cytokines, leading to inflammation-induced myopathy, and SM wasting. This local effect of HF on SM may, on the other hand, contribute to systemic inflammation, as this tissue actively secretes cytokines. Since increasing evidence points out to an anti-inflammatory effect of exercise training, the goal of the present study was to investigate its effect in rats with HF after post-myocardial infarction (MI), with special regard to the expression of TNF-alpha and IL-10 in the soleus and extensor digitorum longus (EDL), muscles with different fiber composition. Wistar rats underwent left thoracotomy with ligation of the left coronary artery, and were randomly assigned to either a sedentary (Sham-operated and MI sedentary) or trained (Sham-operated and MI trained) group. Animals in the trained groups ran on a treadmill (0% grade at 13-20 m/min) for 60 min/day, 5 days/week, for 8-10 weeks. The training protocol was able to reverse the changes induced by MI, decreasing TNF-alpha protein (26%, P<0.05) and mRNA (58%, P<0.05) levels in the soleus, when compared with the sedentary MI group. Training also increased soleus IL-10 expression (2.6-fold, P<0.001) in post-MI HF rats. As a consequence, the IL-10/TNF-alpha ratio was increased. This "anti-inflammatory effect" was more pronounced in the soleus than in the EDL, suggesting a fiber composition dependent response.
心力衰竭（HF）与骨骼肌（SM）的变化有关，这种变化可能是局部促炎（TNF-α）和抗炎（IL-10）细胞因子表达失衡的结果，导致炎症性肌病和 SM 消耗。另一方面，HF 对 SM 的这种局部影响可能会导致全身炎症，因为该组织会主动分泌细胞因子。由于越来越多的证据表明运动训练具有抗炎作用，因此本研究的目的是在心肌梗死后（MI）的 HF 大鼠中研究其对 SM 的影响，特别关注比目鱼肌和伸趾长肌（EDL）中 TNF-α和 IL-10 的表达，这两种肌肉具有不同的纤维组成。Wistar 大鼠接受左开胸术，并结扎左冠状动脉，然后随机分为安静组（假手术和 MI 安静组）或训练组（假手术和 MI 训练组）。训练组动物在跑步机上以 0%的坡度以 13-20 m/min 的速度每天运动 60 分钟，每周运动 5 天。8-10 周后，训练方案能够逆转 MI 引起的变化，与 MI 安静组相比，比目鱼肌中的 TNF-α蛋白（减少 26%，P<0.05）和 mRNA（减少 58%，P<0.05）水平降低。训练还增加了 HF 后 MI 大鼠比目鱼肌的 IL-10 表达（增加 2.6 倍，P<0.001）。因此，IL-10/TNF-α 比值增加。这种“抗炎作用”在比目鱼肌中比在 EDL 中更为明显，表明存在纤维组成依赖性反应。
