Lira F S, Rosa J C, Yamashita A S, Koyama C H, Batista M L, Seelaender M
Molecular Biology of the Cell Group, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1524, CEP 05508-900 Butanta, São Paulo, Brazil.
Cytokine. 2009 Feb;45(2):80-5. doi: 10.1016/j.cyto.2008.10.018. Epub 2008 Dec 20.
White adipose tissue (WAT) is the source of pro- and anti-inflammatory cytokines and recently, it has been recognized as an important source of interleukin 10 (IL-10). Acute physical exercise is known to induce an anti-inflammatory cytokine profile, however, the effect of chronic physical exercise on the production of IL-10 by WAT has never been examined. We assessed IL-10 and TNF-alpha concentration in WAT of rats engaged in endurance training. Animals were randomly assigned to either a sedentary control group (S, n=7) or an endurance trained group (T, n=8). Trained rats ran on a treadmill 5 days/wk for 8 wk (55-65% VO(2max)). Detection of IL-10 and TNF-alpha protein and mRNA expression, as well as the gene expression of PPAR-gamma, and immunocytochemistry to detect mononuclear phagocytes were carried out. A reduction in absolute retroperitoneal adipose tissue (RPAT) weight in T (44%; p<0.01), when compared with S was observed. IL-10 concentration was increased (1.5-fold, p<0.05), to a higher extent than that of TNF-alpha (66%, p<0.05) in the mesenteric adipose tissue (MEAT) of the trained group, while no change related to training was observed in RPAT. In MEAT, IL-10/TNF-alpha ratio was increased in T, when compared with S (30%; p<0.05). PPAR-gamma gene expression was increased in T (1.1-fold; p<0.01), when compared with S in the same adipose depot. No monocyte infiltration was found. In conclusion, exercise training induced increased IL-10 expression in the mesenteric depot, resulting in a modified IL-10/TNF-alpha ratio. We also conclude that WAT presents a depot-specific response to endurance training regarding the studied aspects.
白色脂肪组织（WAT）是促炎和抗炎细胞因子的来源，最近，它被认为是白细胞介素10（IL-10）的重要来源。已知急性体育锻炼会诱导抗炎细胞因子谱，然而，慢性体育锻炼对WAT产生IL-10的影响从未被研究过。我们评估了进行耐力训练的大鼠WAT中IL-10和肿瘤坏死因子-α（TNF-α）的浓度。动物被随机分为久坐对照组（S，n = 7）或耐力训练组（T，n = 8）。训练的大鼠每周5天在跑步机上跑步，持续8周（55 - 65%最大摄氧量（VO₂max））。进行了IL-10和TNF-α蛋白及mRNA表达的检测，以及过氧化物酶体增殖物激活受体-γ（PPAR-γ）的基因表达检测，并通过免疫细胞化学检测单核吞噬细胞。与S组相比，观察到T组的腹膜后脂肪组织（RPAT）绝对重量减少（44%；p < 0.01）。训练组的肠系膜脂肪组织（MEAT）中IL-10浓度升高（1.5倍，p < 0.05），升高幅度高于TNF-α（66%，p < 0.05），而RPAT中未观察到与训练相关的变化。与S组相比，T组MEAT中的IL-10/TNF-α比值升高（30%；p < 0.05）。与同一脂肪库中的S组相比，T组PPAR-γ基因表达升高（1.1倍；p < 0.01）。未发现单核细胞浸润。总之，运动训练诱导肠系膜脂肪库中IL-10表达增加，导致IL-10/TNF-α比值改变。我们还得出结论，就所研究的方面而言，WAT对耐力训练呈现出脂肪库特异性反应。
