CHU Clermont-Ferrand, Department of Pharmacy, Hôp G Montpied, F-63003 Clermont-Ferrand, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):45-50. doi: 10.1016/j.jchromb.2009.11.018.
The anesthetic sevoflurane can now be delivered over periods of up to 48h using a newly developed medical system, the AnaConDa (anesthetic conserving device). Lack of pharmacokinetic data on sevoflurane and its main metabolite (hexafluoroisopropanol, HFIP) in this indication prompted us to develop a headspace GC-MS method to quantify the two substances. The only previously published method for assaying the two substances could not be adapted to our study since it uses expensive and rarely employed system components together with toxic carbon disulfide as a dilution solvent. The method developed is straightforward and uses the relatively non-toxic solvent undecane as dilution solvent and chloroform as internal standard. The method is linear for a concentration range of 1-150microg/ml, and presents high accuracy and precision. LOD and LOQ are 0.2 and 1microg/ml, with a short analysis time (7.6 min for a single analysis). The method was applied to determine the plasma levels of sevoflurane and HFIP in six patients under 48-h anesthetic sedation delivered via the AnaConDa system. Average sevoflurane and HFIP concentrations plateaued at 75 and 4microg/ml, respectively. Sevoflurane quickly tailed off after inhalation was stopped, and HFIP levels remained low.
现在，一种新开发的医疗系统——AnaConDa（麻醉气体储存器），可以在长达 48 小时的时间内输送麻醉剂七氟醚。由于缺乏七氟醚及其主要代谢物（六氟异丙醇，HFIP）在该适应症下的药代动力学数据，我们开发了一种顶空 GC-MS 方法来定量这两种物质。唯一以前发表的测定这两种物质的方法不能适应我们的研究，因为它使用昂贵且很少使用的系统组件，以及有毒的二硫化碳作为稀释溶剂。开发的方法简单直接，使用相对无毒的溶剂十一烷作为稀释溶剂，氯仿作为内标。该方法在 1-150μg/ml 的浓度范围内呈线性，具有较高的准确性和精密度。LOD 和 LOQ 分别为 0.2 和 1μg/ml，分析时间短（单次分析 7.6 分钟）。该方法应用于通过 AnaConDa 系统对 6 名接受 48 小时麻醉镇静的患者进行的血浆中七氟醚和 HFIP 水平的测定。七氟醚和 HFIP 的平均浓度分别稳定在 75μg/ml 和 4μg/ml。吸入停止后，七氟醚迅速衰减，HFIP 水平保持较低。
