Aleksyniene R, Thomsen J S, Eckardt H, Bundgaard K G, Lind M, Hvid I
Orthopaedic Division of Northern Denmark, Aalborg University Hospital, University of Aarhus, Aalborg, Denmark.
J Musculoskelet Neuronal Interact. 2009 Oct-Dec;9(4):268-77.
Since the approval of parathyroid hormone (PTH) as treatment for osteoporosis, PTH has increasingly been investigated for bone repair and regeneration. The aim of the study was to investigate the effects of intermittent PTH treatment on the microstructure of regenerated mineralizing tissue after distraction osteogenesis in rabbits. After tibial mid-diaphyseal osteotomy the callus was distracted 1 mm/day for 10 days. 72 rabbits were divided in to 3 groups, which daily received a PTH (1-34) 25 microg/kg injection for 30 days; a saline injection for 10 days and a PTH injection for 20 days; or a saline injection for 30 days. The microstructure of the regenerate was assessed by micro computed tomography (microCT). In all 51 obtained specimens were evaluated morphometrically using three different volumes of interests. The results showed that treatment with PTH during distraction osteogenesis resulted in a significantly higher trabecular number, a more isotropic trabecular orientation, a higher connectivity density, and a higher mineralizing tissue mass. We also found that distraction calluses treated with PTH were more mature than the non-treated.
treatment with PTH resulted in an enhanced microstructure of the newly regenerated mineralizing tissue indicating that PTH has a potential role as a stimulating agent during distraction osteogenesis.
自从甲状旁腺激素（PTH）被批准用于治疗骨质疏松症以来，人们越来越多地研究其在骨修复和再生方面的作用。本研究的目的是探讨间歇性PTH治疗对兔牵张成骨后再生矿化组织微观结构的影响。在胫骨中1/3骨干截骨术后，骨痂以每天1毫米的速度牵张10天。72只兔子被分为3组，分别接受每天25微克/千克的PTH（1-34）注射，共30天；先注射生理盐水10天，再注射PTH 20天；或注射生理盐水30天。通过微计算机断层扫描（microCT）评估再生组织的微观结构。对所有51个获得的标本使用三种不同的感兴趣体积进行形态计量学评估。结果表明，在牵张成骨过程中使用PTH治疗可导致小梁数量显著增加、小梁取向更各向同性、连通性密度更高以及矿化组织质量更高。我们还发现，用PTH治疗的牵张骨痂比未治疗的更成熟。
PTH治疗可增强新再生矿化组织的微观结构，表明PTH在牵张成骨过程中具有作为刺激剂的潜在作用。
