School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington, Leicestershire, UK.
Prion. 2009 Oct-Dec;3(4):236-9. doi: 10.4161/pri.3.4.10425. Epub 2009 Oct 23.
Prions can be amplified by serial protein misfolding cyclic amplification (sPMCA) from the milk of a high proportion of apparently healthy, scrapie exposed sheep with PRNP genotypes not previously associated with high disease penetrance. These data strongly suggest the widespread presence of subclinical scrapie infections within scrapie-exposed flocks containing sheep with a range of susceptible PRNP genotypes. These data also lead to the hypothesis that similar subclinical disease states may be common for other animal and human prion diseases. Furthermore, the application of sPMCA to milk provides a method to detect such subclinical disease. Here, we describe the high level amplification of bovine spongiform encephalopathy (BSE) prions from both ovine and bovine origin, a methodology that will facilitate the detection of any prions secreted within bovine and ovine milk during subclinical and clinical BSE disease.
朊病毒可通过连续蛋白错误折叠循环扩增（sPMCA）从高比例的表现健康、暴露于瘙痒病的牛奶中扩增，这些牛奶来自先前与高疾病穿透性无关的 PRNP 基因型的绵羊。这些数据强烈表明，在含有一系列易感 PRNP 基因型的瘙痒病暴露羊群中，存在广泛的亚临床瘙痒病感染。这些数据还提出了一个假设，即类似的亚临床疾病状态可能在其他动物和人类朊病毒病中很常见。此外，sPMCA 在牛奶中的应用提供了一种检测此类亚临床疾病的方法。在这里，我们描述了牛海绵状脑病（BSE）朊病毒从绵羊和牛来源的高水平扩增，这种方法将有助于检测亚临床和临床 BSE 疾病期间在牛和羊乳中分泌的任何朊病毒。
