Department of Urology, KEPCO (Korea Electric Power Corporation) Medical Foundation, Hanil General Hospital, Seoul, Korea.
J Korean Med Sci. 2009 Dec;24(6):1139-44. doi: 10.3346/jkms.2009.24.6.1139. Epub 2009 Nov 9.
To evaluate the ability of fluorescence in situ hybridization (FISH) in detecting bladder urothelial carcinoma (BUC), FISH and cytology were compared for the evaluation of 308 consecutive urine samples from patients suspected of having BUC. All patients underwent cystoscopy for identification of bladder lesions. The FISH results were compared with the cytology assessment. In all, 122 patients had confirmed BUC. Among them, 68 (55.7%) were FISH-positive, while only 33 (27%) were positive on cytology. According to disease stage (superficial vs. invasive) and grade (low vs. high), the sensitivities of FISH were also significantly higher than those of cytology in all categories. Moreover, in 36 patients who had no visible tumor with flat, erythematous mucosa (suspicious lesion), FISH was more sensitive than cytology for the detection of BUC (83.3% vs. 33.3%, P=0.002). The FISH was negative in 168 (90.3%) of 186 patients with no histological evidence of BUC or negative cystoscopy findings. The sensitivity of FISH for detecting BUC was superior to that of cytology, regardless of tumor stage and grade. FISH is a significant additional and complementary method for detection of BUC in patients who have suspicious lesions on cystoscopy.
为了评估荧光原位杂交（FISH）检测膀胱尿路上皮癌（BUC）的能力，比较了 FISH 和细胞学检查对 308 例疑似患有 BUC 的患者尿液样本的评估结果。所有患者均接受膀胱镜检查以确定膀胱病变。将 FISH 结果与细胞学评估进行比较。共有 122 例患者确诊为 BUC。其中，68 例（55.7%）FISH 阳性，而仅 33 例（27%）细胞学阳性。根据疾病分期（浅表 vs. 浸润）和分级（低 vs. 高），FISH 在所有类别中的敏感性也明显高于细胞学。此外，在 36 例具有平坦、红斑状黏膜（可疑病变）且无可见肿瘤的患者中，FISH 检测 BUC 的敏感性高于细胞学（83.3% vs. 33.3%，P=0.002）。在 186 例无组织学证据或膀胱镜检查结果阴性的 BUC 患者中，FISH 为阴性（168 例，90.3%）。无论肿瘤分期和分级如何，FISH 检测 BUC 的敏感性均优于细胞学。FISH 是一种重要的附加和补充方法，可用于检测膀胱镜检查中可疑病变患者的 BUC。
