Department of Pulmonary and Critical Care Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
J Korean Med Sci. 2010 Jun;25(6):863-7. doi: 10.3346/jkms.2010.25.6.863. Epub 2010 May 24.
To evaluate the prognostic importance of chromosomal instability (CIN) in squamous cell carcinoma (SCC) of the lung, the relationship between CIN detected by fluorescence in situ hybridization (FISH) and survival in SCC patients was examined. Forty-seven surgical specimens of lung SCC were analyzed. To identify tumors with CIN, p16 and multi-target DNA FISH assays for c-myc, chromosome 6, EGFR, and chromosome 5 (LAVysion, Vysis) were performed on nuclei extracted from paraffin-embedded tumor tissues. Survival rates were compared in terms of age, T factor, N factor, CIN, and smoking status. A sample was defined as CIN-positive if at least four of the five chromosomes were positive. Among the 47 specimens, 9 (19%) were CIN-positive. The overall survival rate was 66%. Overall survival rates were estimated as 33.3% for CIN-positive patients and 76.7% for CIN-negative patients (Hazard ratio 3.47; 95% Confidence interval, 1.25-9.67; P=0.017). In multivariate analysis, the presence of CIN was a predictive factor for survival. CIN-positive based on FISH can be prognostic factor of lung SCC.
为了评估染色体不稳定性（CIN）在肺鳞癌（SCC）中的预后意义，研究了通过荧光原位杂交（FISH）检测到的 CIN 与 SCC 患者生存之间的关系。分析了 47 例肺 SCC 的手术标本。为了鉴定具有 CIN 的肿瘤，从石蜡包埋的肿瘤组织中提取核，进行了 p16 和多靶点 DNA FISH 检测 c-myc、染色体 6、EGFR 和染色体 5（LAVysion、Vysis）。根据年龄、T 因子、N 因子、CIN 和吸烟状况比较了生存率。如果至少有四个五个染色体呈阳性，则将样本定义为 CIN 阳性。在 47 个标本中，有 9 个（19%）为 CIN 阳性。总体生存率为 66%。CIN 阳性患者的总体生存率估计为 33.3%，CIN 阴性患者的总体生存率为 76.7%（风险比 3.47；95%置信区间，1.25-9.67；P=0.017）。在多变量分析中，CIN 的存在是生存的预测因素。基于 FISH 的 CIN 阳性可以是肺 SCC 的预后因素。
