Salomonson Inger, Zou Jinyu, Carlsson Marianne, Bergh Ann, Lundström Fredrik
GE Healthcare Bio-Sciences AB, Uppsala, Sweden.
J Biomol Tech. 2009 Dec;20(5):282-4.
Gel filtration (GF) is an excellent tool to acquire information about sizing (identity), purity, and the multimeric state of a protein of interest. Superdex 200 and Superdex 75 are outstanding GF media for such analysis. To speed up analysis and keep sample and buffer consumption at minimum, two prepacked short GF columns have been developed, Superdex 200 5/150 GL and Superdex 75 5/150 GL. With lengths of only 15 cm and volume of 3 ml, these columns allow rapid analysis (6-12 min/run) with minimal sample (4-50microl) and buffer consumption.
凝胶过滤（GF）是获取有关目标蛋白质大小（特性）、纯度和多聚体状态信息的出色工具。Superdex 200和Superdex 75是用于此类分析的出色凝胶过滤介质。为了加快分析速度并将样品和缓冲液消耗降至最低，已开发出两种预装的短凝胶过滤柱，即Superdex 200 5/150 GL和Superdex 75 5/150 GL。这些柱长度仅为15 cm，体积为3 ml，能够以最少的样品（4 - 50微升）和缓冲液消耗进行快速分析（每次运行6 - 12分钟）。
