National Center for Natural Product Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Planta Med. 2010 May;76(7):743-50. doi: 10.1055/s-0029-1240628. Epub 2009 Nov 30.
Gas chromatography-flame ionization detection (GC-FID) was used to assess the chemical profile and quantification of cannabinoids to identify the differences, if existing, in the chemical constituents of in vitro propagated plants (IVP), conventionally grown plants (VP) and indoor grown mother plants (MP-Indoor) of a high THC yielding variety of Cannabis sativa L. during different developmental stages of growth. In general, THC content in all groups increased with plant age up to a highest level during the budding stage where the THC content reached a plateau before the onset of senescence. The pattern of changes observed in the concentration of other cannabinoids content with plants age has followed a similar trend in all groups of plants. Qualitatively, cannabinoids profiles obtained using GC-FID, in MP-indoor, VP and IVP plants were found to be similar to each other and to that of the field grown mother plant (MP field) of C. sativa. Minor differences observed in cannabinoids concentration within and among the groups were not found to be statistically significant. Our results confirm the clonal fidelity of IVP plants of C. sativa and suggest that the biochemical mechanism used in this study to produce the micropropagated plants does not affect the metabolic content and can be used for the mass propagation of true to type plants of this species for commercial pharmaceutical use.
气相色谱-火焰离子化检测（GC-FID）用于评估大麻素的化学特征和定量分析，以确定体外繁殖植物（IVP）、常规生长植物（VP）和室内生长母本植物（MP-Indoor）在不同生长阶段的化学成分是否存在差异。在高 THC 含量的大麻品种。一般来说，所有组的 THC 含量随着植物年龄的增长而增加，在萌芽阶段达到最高水平，在衰老开始前达到平台期。在所有植物组中，观察到的其他大麻素含量随植物年龄变化的模式也遵循类似的趋势。使用 GC-FID 获得的大麻素图谱，在 MP-Indoor、VP 和 IVP 植物中，与田间生长的母本植物（MP 田间）相似。在组内和组间观察到的大麻素浓度的微小差异没有统计学意义。我们的结果证实了大麻的 IVP 植物的克隆保真度，并表明本研究中用于生产微繁殖植物的生化机制不会影响代谢含量，并可用于该物种的真实类型植物的大规模繁殖，用于商业制药用途。
