采用离子液体双相溶剂系统修饰剂的高速逆流色谱法从知母中制备分离新芒果苷和芒果苷。
Preparative isolation of neomangiferin and mangiferin from Rhizoma anemarrhenae by high-speed countercurrent chromatography using ionic liquids as a two-phase solvent system modifier.
机构信息
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong, PR China.
出版信息
J Sep Sci. 2010 Jan;33(1):31-6. doi: 10.1002/jssc.200900528.
A preparative high-speed countercurrent chromatography method for isolation and purification of neomangiferin and mangiferin from Rhizoma anemarrhenae was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Neomangiferin and mangiferin were purified from the crude extract of R. anemarrhenae by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2 v/v) as two-phase solvent system. In total, 22.5 mg of neomangiferin and 70.6 mg of mangiferin were obtained from 150 mg of the crude extract. The purities of neomangiferin and mangiferin were 97.2 and 98.1%, respectively, as determined by HPLC. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.
成功建立了一种使用离子液体作为两相溶剂系统改性剂的制备性高速逆流色谱法，用于从知母根茎中分离和纯化新芒果苷和芒果苷。新芒果苷和芒果苷从知母粗提取物中用乙酸乙酯-水-[C（4）mim][PF（6）]（5:5:0.2v/v）作为两相溶剂系统进行纯化。总共从 150mg 粗提取物中获得了 22.5mg 的新芒果苷和 70.6mg 的芒果苷。通过 HPLC 确定新芒果苷和芒果苷的纯度分别为 97.2%和 98.1%。通过（1）H-NMR 和（13）C-NMR 鉴定了分离化合物的化学结构。
Zotero 插件