采用离子液体修饰双相溶剂体系的高速逆流色谱法从黄兰花中制备性分离黄酮类化合物。

Preparative isolation of flavonoid compounds from Oroxylum indicum by high-speed counter-current chromatography by using ionic liquids as the modifier of two-phase solvent system.

机构信息

College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, PR China.

出版信息

J Sep Sci. 2010 Apr;33(8):1058-63. doi: 10.1002/jssc.200900612.

DOI:10.1002/jssc.200900612

PMID:20175085

Abstract

A preparative high-speed counter-current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Two flavonoid compounds including baicalein-7-O-diglucoside and baicalein-7-O-glucoside were purified from the crude extract of O. indicum by using ethyl acetate-water-[C(4)mim][PF(6)] (5:5:0.2, v/v) as two-phase solvent system. 36.4 mg of baicalein-7-O-diglucoside and 60.5 mg of baicalein-7-O-glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by (1)H-NMR and (13)C-NMR.

摘要

成功建立了一种使用离子液体作为两相溶剂系统改性剂的从黄桐中分离和纯化类黄酮化合物的制备高速逆流色谱法。从黄桐粗提取物中，使用乙酸乙酯-水-[C（4）mim][PF（6）]（5:5:0.2，v/v）作为两相溶剂系统，分离并纯化了两种包括黄芩素-7-O-二葡萄糖苷和黄芩素-7-O-葡萄糖苷的类黄酮化合物。从 120 毫克粗提取物中获得了 36.4 毫克黄芩素-7-O-二葡萄糖苷和 60.5 毫克黄芩素-7-O-葡萄糖苷。它们的纯度分别通过 HPLC 面积归一化法确定为 98.7%和 99.1%。通过（1）H-NMR 和（13）C-NMR 确定了分离化合物的化学结构。