Long Wei-Fu, Li Liang, Chen Huai-Qing, Tang Yong, He Xue-Ling, Jing Rong-Zhou
Biomedical Engneering Institute, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Sep;40(5):843-7.
To investigate the application of metabonomics in research of osteoporosis, through detecting change of the endogenous metabolites in plasma from osteoporotic rats by ovariectomy.
Six old-months female SD rats were randomly divided into sham and OVX group. Fifth month after ovariectomy, plasma were collected from both groups, respectively. The metabolic profiles were investigated using 1H-NMR spectroscopy of plsama combined with pattern recognition techniques, including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA).
The PCA PLS-DA plots of the plasma samples presented marked clustering between sham group and OVX group. Compared to sham group, the level of low molecular metabolites such as lactate, acetone and ethonal were higer, glucose, choline/phosphatidylcholine (Cho/PC), alanine (Ala), high density lipoprotein/low density lipoprotein (HDL/LDL), very low density lipoprotein/low density lipoprotein, fatty acid and glucose were lower.
Obvious changes in metabonomics of plasma from osteoporotic rats were observed.
通过检测去卵巢骨质疏松大鼠血浆中内源性代谢物的变化，探讨代谢组学在骨质疏松研究中的应用。
将6只6月龄雌性SD大鼠随机分为假手术组和去卵巢组。去卵巢术后第5个月，分别采集两组大鼠的血浆。采用血浆1H-NMR光谱结合模式识别技术（包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA））研究代谢谱。
血浆样本的PCA和PLS-DA图显示假手术组和去卵巢组之间有明显聚类。与假手术组相比，乳酸、丙酮和乙醇等小分子代谢物水平升高，葡萄糖、胆碱/磷脂酰胆碱（Cho/PC）、丙氨酸（Ala）、高密度脂蛋白/低密度脂蛋白（HDL/LDL）、极低密度脂蛋白/低密度脂蛋白、脂肪酸和葡萄糖水平降低。
观察到骨质疏松大鼠血浆代谢组学有明显变化。
