Harri Minna, Svoboda Peter, Mori Toshiaki, Mutanen Pertti, Kasai Hiroshi, Savela Kirsti
Finnish Institute of Occupational Health, Helsinki, Finland.
Free Radic Res. 2005 Sep;39(9):963-72. doi: 10.1080/10715760500190115.
Oxidative DNA damage and repair, as measured by 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine and DNA samples were studied in association with work-related diesel exhaust exposure among garage and waste collection workers. Seasonal variations of the urinary 8-OHdG levels in pre- and two post-workshift urine samples of 29 exposed workers and 36 control persons were evaluated. The mean+/-SE levels of post-workshift 8-OHdG (mumol/mol crea) were 1.52+/-0.44 in winter and 1.61+/-0.33 in summer for the exposed workers, and 1.56+/-0.61 in winter and 1.43+/-0.49 in summer for the controls, respectively. No significant difference in the urinary 8-OHdG levels between exposed workers and control subjects in winter (p=0.923) and summer (p=0.350) was observed. A linear mixed model, adjusted for years of employment, age, ex/non-smoking and BMI, indicated no significant dose exposure-relationships between the urinary 8-OHdG and 15 PAH air concentrations nor between the 8-OHdG and 7 PAH monohydroxy-metabolites analyzed in the same workers. 8-OHdG was also analyzed in the mononuclear cell DNA of 19 exposed and 18 control subjects. The mean value of 8-OHdG/non-modified 2'-deoxyguanosine (8-OHdG/105 dG+/-SE) were 4.89+/-0.17 for the exposed and 4.11+/-0.16 for the control persons, which showed no correlation with the urinary 8-OHdG levels (r=0.01, n=28, P=0.96). The PAH exposure at workplaces was mainly composed of volatile compounds, particularly naphthalene, suggesting low exposure through the respiratory tract and a low effect of PAH in ROS induction.
通过检测尿液和DNA样本中的8-羟基-2'-脱氧鸟苷（8-OHdG）来研究氧化DNA损伤与修复，并将其与汽车修理厂和垃圾收集工人工作中接触柴油废气的情况相关联。评估了29名暴露工人和36名对照人员班前和两个班后尿液样本中尿8-OHdG水平的季节性变化。暴露工人班后8-OHdG（μmol/mol肌酐）的平均±标准误水平在冬季为1.52±0.44，夏季为1.61±0.33；对照人员冬季为1.56±0.61，夏季为1.43±0.49。在冬季（p = 0.923）和夏季（p = 0.350），暴露工人与对照人员的尿8-OHdG水平均未观察到显著差异。采用线性混合模型，对就业年限、年龄、吸烟/不吸烟和体重指数进行校正后，结果表明尿8-OHdG与15种多环芳烃空气浓度之间以及8-OHdG与在同一工人中分析的7种多环芳烃单羟基代谢产物之间均无显著的剂量暴露关系。还对19名暴露者和18名对照人员的单核细胞DNA进行了8-OHdG分析。暴露者的8-OHdG/未修饰的2'-脱氧鸟苷（8-OHdG/105 dG±标准误）平均值为4.89±0.17，对照人员为4.11±0.16，与尿8-OHdG水平无相关性（r = 0.01，n = 28，P = 0.96）。工作场所的多环芳烃暴露主要由挥发性化合物组成，尤其是萘，这表明通过呼吸道的暴露较低，且多环芳烃在活性氧诱导中的作用较小。
