Chang Fu-Kuei, Mao I-Fang, Chen Mei-Lien, Cheng Shu-Fang
Department of Health Management, College of Medical Science, I-Shou University, Yanchao Township, Kaohsiung County, Taiwan.
Ann Occup Hyg. 2011 Jun;55(5):519-25. doi: 10.1093/annhyg/mer010. Epub 2011 Mar 23.
This study assessed the relationships between ethylbenzene exposure and levels of 8-hydroxydeoxyguanosine (8-OHdG) among spray painters. Sixty-four male workers employed at a large shipyard were recruited for this investigation. Fifteen spray painters exposed to paint, together with two non-exposed groups, namely 19 sandblasting workers and 30 office staffs were selected as the subjects. Personal exposure to xylene and ethylbenzene in air were collected using diffusive samplers. Urine samples of the spray painters were collected after a month-long holiday leave and during the pre- and post-workshifts. Urine samples of sandblasting workers and office staffs were gathered after their shift. Urinary mandelic acid and methyl hippuric acid were used as biological indices of dose of ethylbenzene and xylene, respectively. Urinary 8-OHdG was used as biomarker of oxidative DNA damage. The post-workshift concentration of urinary 8-OHdG for 10 spray painters (30.3 ± 9.28 μg g(-1) creatinine) significantly exceeded that of holiday leave (7.20 ± 1.08 μg g(-1) creatinine; P = 0.001). The post-workshift concentration of urinary 8-OHdG was higher among 15 spray painters (29.0 ± 6.52 μg g(-1) creatinine) than sandblasting workers (9.14 ± 2.05 μg g(-1) creatinine; P = 0.01) and office staffs (8.35 ± 0.84 μg g(-1) creatinine; P = 0.007). A stepwise regression model revealed an 8.11 μg g(-1) creatinine increase per 1 p.p.m. increase in ethylbenzene [95% confidence interval (CI) 4.13-12.1]. A stepwise regression model revealed an increase of 6.04 μg g(-1) creatinine (95% CI 2.23-9.84) per 1 p.p.m. in ethylbenzene after adjustment of age (95% CI 2.23-9.84). This pilot study suggests that occupational exposure to paint increases oxidative DNA injury. Moreover, urinary 8-OHdG levels displayed greater DNA damage in spray painters compared to other unexposed groups and their holiday leave samples. A significant correlation was found between urinary 8-OHdG and the exposure to ethylbenzene. The ethylbenzene exposure could not explain all urinary 8-OHdG measured. Other components of paint deserve further investigation.
本研究评估了喷漆工人接触乙苯与8-羟基脱氧鸟苷（8-OHdG）水平之间的关系。招募了一家大型造船厂的64名男性工人参与此次调查。选取了15名接触油漆的喷漆工人，以及两个未接触组，即19名喷砂工人和30名办公室职员作为研究对象。使用扩散采样器收集空气中个人接触二甲苯和乙苯的情况。喷漆工人的尿液样本在为期一个月的假期后以及班前和班后采集。喷砂工人和办公室职员的尿液样本在轮班后收集。尿中扁桃酸和甲基马尿酸分别用作乙苯和二甲苯剂量的生物学指标。尿8-OHdG用作氧化DNA损伤的生物标志物。10名喷漆工人班后尿8-OHdG浓度（30.3±9.28μg g⁻¹肌酐）显著高于假期浓度（7.20±1.08μg g⁻¹肌酐；P = 0.001）。15名喷漆工人班后尿8-OHdG浓度（29.0±6.52μg g⁻¹肌酐）高于喷砂工人（9.14±2.05μg g⁻¹肌酐；P = 0.01）和办公室职员（8.35±0.84μg g⁻¹肌酐；P = 0.007）。逐步回归模型显示，乙苯每增加1 ppm，尿8-OHdG浓度增加8.11μg g⁻¹肌酐[95%置信区间（CI）4.13 - 12.1]。调整年龄后，逐步回归模型显示乙苯每增加1 ppm，尿8-OHdG浓度增加6.04μg g⁻¹肌酐（95% CI 2.23 - 9.84）。这项初步研究表明，职业性接触油漆会增加氧化DNA损伤。此外，与其他未接触组及其假期样本相比，喷漆工人的尿8-OHdG水平显示出更大的DNA损伤。尿8-OHdG与乙苯接触之间存在显著相关性。乙苯接触无法解释所测的所有尿8-OHdG情况。油漆的其他成分值得进一步研究。
