Li Ting, Zhou Jianhua, Deng Zhenghao, Fu Chunyan, Jiang Haiying, Gao Zhenqin, Wang Jinsheng, Ren Hongzheng, Wang Peng
Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2009 Nov;34(11):1114-9.
To investigate the expression of fibroblast growth factor-2 (FGF-2) and osteopontin (OPN) in non-small cell lung cancer (NSCLC) tissues and analyze the correlation between FGF-2 and OPN.
Immunohistochemical SP method was used to detect the expression of FGF-2 and OPN in 76 patients with NSCLC and 15 normal lung tissues. The effect of FGF-2 on OPN expression at mRNA and protein level in A549 cell was examined by RT-PCR and Western blot.
The positive expression of FGF-2 (65.8%) and OPN (60.5%) in the NSCLC tissues was significantly higher than that in the normal lung tissues (13.3% and 0, respectively ) (P<0.01). The expression of FGF-2 and OPN was closely related to TNM stages and the lymph node metastasis (all Ps<0.01), but not to histological types, sex, and age of NSCLC patients (all Ps>0.05).A positive correlation was found between the expression of FGF-2 and OPN in NSCLC (r=0.552,P<0.01). The expression of OPN protein and mRNA was up-regulated by FGF-2 in A549 cells.
The overexpression of FGF-2 and OPN is related to the metastasis and invasion of NSCLC.FGF-2 may promote the metastasis and invasion of NSCLC depending on the upregulation of OPN expression.
探讨成纤维细胞生长因子-2（FGF-2）和骨桥蛋白（OPN）在非小细胞肺癌（NSCLC）组织中的表达情况，并分析FGF-2与OPN之间的相关性。
采用免疫组织化学SP法检测76例NSCLC患者及15例正常肺组织中FGF-2和OPN的表达。通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blot）检测FGF-2对A549细胞中OPN在mRNA和蛋白质水平表达的影响。
NSCLC组织中FGF-2的阳性表达率（65.8%）和OPN的阳性表达率（60.5%）显著高于正常肺组织（分别为13.3%和0）（P<0.01）。FGF-2和OPN的表达与TNM分期及淋巴结转移密切相关（所有P值<0.01），但与NSCLC患者的组织学类型、性别及年龄无关（所有P值>0.05）。NSCLC中FGF-2与OPN的表达呈正相关（r=0.552，P<0.01）。FGF-2可上调A549细胞中OPN蛋白和mRNA的表达。
FGF-2和OPN的过表达与NSCLC的转移和侵袭有关。FGF-2可能通过上调OPN的表达促进NSCLC的转移和侵袭。
