[非小细胞肺癌中特异性富含AT序列结合蛋白mRNA的表达及其临床病理意义]
[Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer].
作者信息
Zhou Lai-Yong, Liu Fang, Tong Jian, Chen Qun-Qing, Zhang Fu-Wei
机构信息
Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):534-7.
OBJECTIVE
To detect the expression of AT-rich sequence-binding protein (SATB1) mRNA in non-small cell lung cancer (NSCLC) and explore the role of SATB1 in the development of NSCLC.
METHODS
The total RNA was extracted from NSCLC tissues and normal lung tissues and reverse transcribed into cDNA. Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of SATB1 mRNA these tissues.
RESULTS
The expression of SATB1 mRNA was 13-fold higher in NSCLC tissues than in normal lung tissues (P<0.001), and in metastatic and nonmetastatic NSCLC, the expression was 23.63 and 5.57 folds that in normal lung tissues, respectively.
CONCLUSION
SATB1 mRNA expression might be associated with the development and lymph node metastasis of NSCLC and may potentially used as an indicator for predicting the prognosis of NSCLC.
目的
检测富含AT序列结合蛋白（SATB1）mRNA在非小细胞肺癌（NSCLC）中的表达，探讨SATB1在NSCLC发生发展中的作用。
方法
提取NSCLC组织和正常肺组织的总RNA，并逆转录为cDNA。采用实时荧光定量RT-PCR检测这些组织中SATB1 mRNA的表达。
结果
NSCLC组织中SATB1 mRNA的表达水平比正常肺组织高13倍（P<0.001），在伴有淋巴结转移和无淋巴结转移的NSCLC组织中，其表达水平分别是正常肺组织的23.63倍和5.57倍。
结论
SATB1 mRNA表达可能与NSCLC的发生发展及淋巴结转移相关，有望作为预测NSCLC预后的指标。
Zotero 插件