Shokri Hojjatollah, Khosravi Alireza, Rad Mohammadali, Jamshidi Shahram
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran 14155-6453, Iran.
J Vet Med Sci. 2010 Mar;72(3):293-6. doi: 10.1292/jvms.09-0421. Epub 2009 Dec 1.
The yeasts of the Malassezia genus are opportunistic microorganisms in the skin and auricular canal of human and animals, mainly cats, and can cause otitis externa and dermatitis disorders. The aim of this study was to evaluate the occurrence of different species of Malassezia in the external ear canal of cats with and without otitis externa. Thirty-one normal cats and 82 animals with otitis externa were clinically examined. Sterile cotton swabs were used to collect specimens from the external ear canal and streaked onto the surface of Sabouraud dextrose agar (SDA) and modified Dixon agar. Malassezia yeasts were isolated from 95.1% and 48.4% of the cats with and without otitis externa, respectively. The rate of isolation in affected animals versus normals was highly significant (P<0.05). Out of the 137 isolates obtained from cats with otitis, 57.7% were identified as M. pachydermatis (with significant frequency; P<0.05), 15.4% as M. obtusa, 11.4% as M. globosa, 7.3% as M. slooffiae, 4.1% as M. sympodialis, 2.4% as M. furfur and 1.6% as M. restricta. Malassezia species were frequently isolated from subjects with age range from 1 to 4 years old (42.7%). Our finding of Malassezia isolates indicated that feline otitis externa can be associated with lipid-dependent Malassezia species in addition to the non lipid- dependent species M. pachydermatis.
马拉色菌属酵母菌是人和动物（主要是猫）皮肤及耳道内的机会性微生物，可引发外耳炎和皮炎疾病。本研究旨在评估患有和未患外耳炎的猫外耳道中不同马拉色菌种的出现情况。对31只正常猫和82只患有外耳炎的动物进行了临床检查。使用无菌棉拭子从外耳道采集标本，并划线接种于沙氏葡萄糖琼脂（SDA）和改良狄克逊琼脂表面。分别从95.1%患有外耳炎的猫和48.4%未患外耳炎的猫中分离出马拉色菌酵母菌。患病动物与正常动物的分离率差异极显著（P<0.05）。从患有外耳炎的猫身上获得的137株分离株中，57.7%被鉴定为厚皮马拉色菌（频率显著；P<0.05），15.4%为钝形马拉色菌，11.4%为球形马拉色菌，7.3%为斯洛菲马拉色菌，4.1%为合轴马拉色菌，2.4%为糠秕马拉色菌，1.6%为限制马拉色菌。马拉色菌种常从1至4岁的个体中分离得到（42.7%）。我们对马拉色菌分离株的研究结果表明，除了非脂质依赖性的厚皮马拉色菌外，猫外耳炎可能还与脂质依赖性马拉色菌种有关。
