Hwang In Koo, Chung Jin Young, Yoo Dae Young, Yi Sun Shin, Youn Hwa Young, Seong Je Kyung, Yoon Yeo Sung
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
J Vet Med Sci. 2010 Mar;72(3):279-84. doi: 10.1292/jvms.09-0374. Epub 2009 Dec 1.
We compared the effects of acupuncture and electroacupuncture on cell proliferation and neuroblast differentiation using specific markers, Ki67 and doublecortin (DCX), in the subgranular zone of the dentate gyrus (SZDG) in 13-week old Wistar rats. Acupuncture and electroacupuncture were applied simultaneously in the acu-points, ST36 (Zusanli) and GV20 (Baihui), once a day for 3 weeks. Acupuncture and electroacupuncture at these acu-points significantly increased the number of Ki67-positive cells and DCX-immunoreactive neuroblasts compared to the control or sham acupuncture group. Electroacupuncture treatment significantly increased the number of well-developed (tertiary) dendrites in the SZDG compared to acupuncture treatment. These results suggest that both acupuncture and electroacupuncture increase neurogenesis in the normal, but that electroacupuncture has greater effects on neuroblast plasticity than acupuncture in the dentate gyrus.
我们使用特异性标志物Ki67和双皮质素（DCX），比较了针刺和电针刺激对13周龄Wistar大鼠齿状回颗粒下区（SZDG）细胞增殖和神经母细胞分化的影响。针刺和电针同时作用于穴位足三里（ST36）和百会（GV20），每天1次，持续3周。与对照组或假针刺组相比，针刺和电针刺激这些穴位显著增加了Ki67阳性细胞和DCX免疫反应性神经母细胞的数量。与针刺治疗相比，电针治疗显著增加了SZDG中发育良好（三级）树突的数量。这些结果表明，针刺和电针刺激均可促进正常情况下的神经发生，但在齿状回中，电针刺激对神经母细胞可塑性的影响比针刺更大。
