Stadnicka A
Gegenbaurs Morphol Jahrb. 1977;123(2):275-86.
Granulosa and theca interna cells were isolated from bovine preovulatory ovarian follicles. They were cultured separately but in the same conditions of cell culture. Both cell types, grown as monolayers, were investigated histochemically with special regard to the activity of several hydroxysteroid dehydrogenases: delta53betaOH-SDH, 17betaOH-SDH, 20alphaOH-SDH and G6P-DH. Bovine granulosa and theca interna cells during in vitro culture showed high activity of delta53betaOH-SDH and G6P-DH, the enzymes essential to progesterone biosynthesis. Enzyme pattern of cultured cells indicated continuation in vitro of luteinization, which in the normal preovulatory follicle of the bovine ovary begins prior to ovulation. There was investigated as well the influence of single doses of gonadotrophic hormones and estradiol on growth, lipid contents and enzymic activity of cultured in vitro bovine granulosa and theca interna cells.
从牛排卵前卵巢卵泡中分离出颗粒细胞和卵泡内膜细胞。它们分别培养，但处于相同的细胞培养条件下。两种细胞类型均以单层生长，针对几种羟基类固醇脱氢酶的活性进行了组织化学研究：δ5-3βOH-SDH、17βOH-SDH、20αOH-SDH和G6P-DH。体外培养期间，牛颗粒细胞和卵泡内膜细胞显示出δ5-3βOH-SDH和G6P-DH的高活性，这两种酶是孕酮生物合成所必需的。培养细胞的酶谱表明在体外黄体化仍在继续，而在牛卵巢正常排卵前卵泡中，黄体化在排卵前就已开始。还研究了单剂量促性腺激素和雌二醇对体外培养的牛颗粒细胞和卵泡内膜细胞生长、脂质含量和酶活性的影响。
