Stadnicka A
Acta Histochem. 1976;57(2):291-301. doi: 10.1016/S0065-1281(76)80056-6.
Histochemical activity of hydroxysteroid dehydrogenases as well as alkaline and acid phosphatase was investigated in porcine granulosa cells cultured in vitro. Granulosa cells, isolated from preovulatory procine ovarian follicles, during in vitro culture showed activity of enzymes participating in steroid biosynthesis. High activity of delta53betta0H-SDH and G6P-DH as well as the activity of alkaline phosphatase, appearing in the course of culture, could be evidence of progressive luteinization of the cells. Activity of 17beta0H-SDH was lower and exhibited strong fluctuations, similarly low was 20xOH-SDH. Gonadotropic hormones caused the increase of synthesis and accumulation of intracellular lipids. They stimulated alkaline and acid phosphatase, and also the activity of the dehydrogenases. LH had the most visible effect. Estradiol stimulated the activity of acid but not alkaline phosphatase and was not influencing, even lowering the activity of dehydrogenases.
对体外培养的猪颗粒细胞中羟基类固醇脱氢酶以及碱性和酸性磷酸酶的组织化学活性进行了研究。从排卵前猪卵巢卵泡中分离出的颗粒细胞，在体外培养期间显示出参与类固醇生物合成的酶的活性。培养过程中出现的δ53β0H - SDH和G6P - DH的高活性以及碱性磷酸酶的活性，可能是细胞进行性黄体化的证据。17β0H - SDH的活性较低且波动较大，20xOH - SDH的活性同样较低。促性腺激素导致细胞内脂质合成和积累增加。它们刺激碱性和酸性磷酸酶以及脱氢酶的活性。促黄体生成素（LH）的作用最为明显。雌二醇刺激酸性磷酸酶的活性，但不刺激碱性磷酸酶的活性，甚至会降低脱氢酶的活性。
