Stadnicka A, Stoklosowa S
Z Mikrosk Anat Forsch. 1976;90(3):458-68.
By means of histochemical technique the activities of delta53beta-, 17beta-, 20alpha-hydroxysteroid dehydrogenases and glucose-6-phosphate dehydrogenase, as well as alkaline and acid phosphatase were investigated in monolayer cultures of theca interna cells, isolated from preovulatory porcine ovarian follicles. It was found that theca interna cells exhibited high and constant activity of delta53beta-hydroxysteroid dehydrogenase and G6P-DH, whereas activities of both 17beta- and 20alpha-hydroxysteroid dehydrogenase were lower and showed some fluctuations during in vitro culture. Addition of LH to the medium brought about the increase of all studied dehydrogenases. FSH was less effective. Estradiol showed quite and inhibiting effect. All the hormones mentioned above caused the increase of alkaline and acid phosphatase activity in cultured porcine theca interna cells.
采用组织化学技术，对从排卵前猪卵巢卵泡分离的卵泡内膜细胞单层培养物中的δ53β -、17β -、20α -羟基类固醇脱氢酶和葡萄糖 - 6 -磷酸脱氢酶的活性，以及碱性磷酸酶和酸性磷酸酶进行了研究。结果发现，卵泡内膜细胞表现出高且恒定的δ53β -羟基类固醇脱氢酶和葡萄糖 - 6 -磷酸脱氢酶活性，而17β -和20α -羟基类固醇脱氢酶的活性较低，并且在体外培养期间表现出一些波动。向培养基中添加促黄体生成素（LH）导致所有研究的脱氢酶活性增加。促卵泡生成素（FSH）的效果较差。雌二醇表现出明显的抑制作用。上述所有激素均导致培养的猪卵泡内膜细胞中碱性磷酸酶和酸性磷酸酶活性增加。
