Department of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
Parasitol Res. 2010 Jan;106(2):493-8. doi: 10.1007/s00436-009-1691-7. Epub 2009 Dec 2.
This is a molecular epidemiological investigation on Theileria equi, a causative agent of equine piroplasmosis. Blood samples were collected from 127 horses from different geographical locations in Sudan. The small subunit of rRNA gene (18S; ~1,600 bp) was amplified from 20 positive field samples and subsequently subjected to direct sequencing and analysis to reveal possible strain differences and the presence of a novel species or genotypes. Sequences were compared with published sequences mainly from South African and Spanish isolates. Eleven distinct T. equi sequences within 18S rRNA gene were identified to have occurred, and three genotypes were lying within the three previously identified groups. Alignments demonstrated extensive sequence variation in the hypervariable region of the 18S rRNA gene and many SNPs within the Sudanese T. equi isolates.
这是一项关于马媾疫锥虫（致马梨形虫病的病原体）的分子流行病学调查。从苏丹不同地理位置采集了 127 匹马的血液样本。从小亚基 rRNA 基因（18S；~1600bp）中扩增了 20 个阳性野外样本，并随后进行直接测序和分析，以揭示可能的菌株差异以及新型种或基因型的存在。将序列与主要来自南非和西班牙分离株的已发表序列进行了比较。在 18S rRNA 基因中发现了 11 种不同的马媾疫锥虫序列，其中 3 种基因型属于之前已鉴定的 3 个组。比对显示，18S rRNA 基因的高变区和苏丹马媾疫锥虫分离株中的许多 SNP 存在广泛的序列变异。
