Lucas M, Solano F, Sanz A
Dpto de Bioquímica, Hospital Universitario V. Macarena, Facultad de Medicina, Sevilla, Spain.
FEBS Lett. 1991 Feb 11;279(1):19-20. doi: 10.1016/0014-5793(91)80239-y.
The apoptosis of human peripheral blood lymphocytes was analyzed by the breakdown of DNA into oligonucleosome-sized fragments. The mature lymphocytes were rendered sensitive to apoptosis by either the omission of fetal bovine serum in the culture medium or the addition of polymyxin B. In the first case it was counteracted by phorbol myristate acetate. The possible involvement of protein kinase C in cell survival is pointed out.
通过将DNA分解为寡核小体大小的片段来分析人外周血淋巴细胞的凋亡。通过在培养基中省略胎牛血清或添加多粘菌素B，使成熟淋巴细胞对凋亡敏感。在第一种情况下，它被佛波醇肉豆蔻酸酯乙酸盐抵消。指出了蛋白激酶C可能参与细胞存活。
