Sanchez V, Lucas M, Sanz A, Goberna R
Departamento de Bioquímica Médica y Biologia Molecular, Hospital Universitario Virgen Macarena, Facultad de Medicina, Sevilla, Spain.
Biosci Rep. 1992 Jun;12(3):199-206. doi: 10.1007/BF01121789.
Apoptosis of freshly isolated rat hepatocytes was induced by either the omission of fetal bovine serum in the culture medium or addition of the protein kinase C inhibitors polymyxin B or staurosporine. The time-course of DNA breakdown into oligonucleosome-sized fragments and the activity of protein kinase C was determined. Hepatocytes were found to be sensitive to bleomycin which induced a high degree of DNA breakdown even within 30 min incubation. Both staurosporine and polymyxin B induced DNA degradation in hepatocytes after three hours incubation, an effect that was partially prevented by phorbol myristate acetate (PMA). After eight hours incubation, PMA failed to counteract this action and itself produced the apoptosis of rat hepatocytes. The results suggest the involvement of protein kinase C in hepatocyte survival.
通过在培养基中省略胎牛血清或添加蛋白激酶C抑制剂多粘菌素B或星形孢菌素，诱导新鲜分离的大鼠肝细胞凋亡。测定了DNA降解为寡核小体大小片段的时间进程以及蛋白激酶C的活性。发现肝细胞对博来霉素敏感，即使在孵育30分钟内，博来霉素也能诱导高度的DNA降解。孵育三小时后，星形孢菌素和多粘菌素B均诱导肝细胞中的DNA降解，佛波醇肉豆蔻酸酯乙酸盐（PMA）可部分阻止这种作用。孵育八小时后，PMA无法抵消这种作用，其本身反而导致大鼠肝细胞凋亡。结果表明蛋白激酶C参与肝细胞的存活。
