Sakano T, Hyodo S, Kittaka E, Tanaka Y, Usui T
Clin Exp Immunol. 1982 Sep;49(3):665-9.
Human peripheral blood lymphocytes were examined for rosette formation with autologous erythrocytes. When normal human lymphocytes were stimulated with phorbol myristate acetate (PMA) in the presence of autologous serum, the levels of autorosette forming cells (ARFC) were strongly enhanced. Pre-culture was necessary for the generation of ARFC by PMA and the maximal level of ARFC was observed at 72 hr of culture. ARFC appear to belong to a T cell subset and the induction of ARFC by PMA was noted in monocyte depleted lymphocyte fractions, indicating monocyte independency.
检测人外周血淋巴细胞与自身红细胞的玫瑰花结形成情况。当正常人淋巴细胞在自身血清存在的情况下用佛波酯（PMA）刺激时，自身玫瑰花结形成细胞（ARFC）的水平会显著增强。PMA诱导ARFC产生需要进行预培养，并且在培养72小时时观察到ARFC的最高水平。ARFC似乎属于T细胞亚群，在去除单核细胞的淋巴细胞组分中观察到PMA对ARFC的诱导作用，表明其诱导过程不依赖单核细胞。
