Biomedical and Tissue Engineering, University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong.
Phytother Res. 2010 Jun;24 Suppl 2:S155-60. doi: 10.1002/ptr.3049.
The objective of the study is to compare the amount of new bone produced by Buguzhi (Psoralea corylifolia fruit) extract in collagen matrix to that produced and collagen matrix in vivo. Eighteen bone defects, 5 mm by 10 mm, were created in the parietal bone of 9 New Zealand white rabbits. Six defects were grafted with Buguzhi extract mixed with collagen matrix. Six defects were grafted with collagen matrix alone (positive control) and 6 were left empty (negative control). Animals were sacrificed on day 14 and the defects were dissected and prepared for histological assessment. Quantitative analysis of new bone formation and bone cells was made on 100 sections (50 sections for each group) using image analysis. A total of 275% more new bone was present in defects grafted with Buguzhi extract in collagen matrix than those grafted with collagen matrix. No bone was formed in the negative control group. The amount of bone cells was also significantly greater in the Buguzhi group than in the positive control group. To conclude, Buguzhi extract in collagen matrix has the effect of increasing new bone formation locally in vivo. Buguzhi extract in collagen matrix can be used as a bone graft material.
本研究旨在比较骨碎补（补骨脂）提取物在胶原基质中与胶原基质在体内产生的新骨量。在 9 只新西兰白兔的顶骨上创建了 18 个 5mm×10mm 的骨缺损。6 个缺损用骨碎补提取物混合胶原基质移植，6 个缺损仅用胶原基质移植（阳性对照），6 个缺损为空（阴性对照）。第 14 天处死动物，解剖缺损并准备进行组织学评估。使用图像分析对 100 个切片（每组 50 个切片）进行新骨形成和骨细胞的定量分析。用骨碎补提取物混合胶原基质移植的缺损中存在的新骨量比仅用胶原基质移植的缺损多 275%。阴性对照组无骨形成。骨碎补组的骨细胞数量也明显多于阳性对照组。总之，骨碎补提取物在胶原基质中具有在体内局部增加新骨形成的作用。骨碎补提取物在胶原基质中可用作骨移植材料。
