Chen Qinhua, Li Yuan, Chen Zilin
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
J Pharm Anal. 2012 Apr;2(2):143-151. doi: 10.1016/j.jpha.2011.11.005. Epub 2011 Nov 26.
The qualitative and quantitative analysis of active constituents in is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins, benzofuran glycosides, flavonoids, and meroterpene were identified. The determination of four major constituents including bavachin, isobavachalcone, bavachinin, and bakuchiol was accomplished by HPLC with UV, MS, and electrochemical detection (ECD). These methods were evaluated for a number of validation characteristics (repeatability, LOD, calibration range, and recovery). ECD obtained a high sensitivity for analysis of the four components; MS provided a high selectivity and sensitivity for determination of bavachin, isobavachalcone, and bavachinin in negative-ion mode. After optimization of the methods, separation, identification. and quantification of the four components in were comprehensively tested by HPLC with UV, MS, and ECD.
采用高效液相色谱（HPLC）结合不同检测方法对[具体物质名称未给出]中的活性成分进行了定性和定量分析。通过HPLC与离子阱质谱（IT-MS）联用对[具体物质名称未给出]的提取物进行了检测，鉴定出18种主要的香豆素、苯并呋喃糖苷、黄酮类化合物和半萜类化合物。采用HPLC结合紫外（UV）、质谱（MS）和电化学检测（ECD）对补骨脂素、异补骨脂查尔酮、补骨脂宁和补骨脂酚这四种主要成分进行了测定。对这些方法的一些验证特性（重复性、检测限、校准范围和回收率）进行了评估。ECD对这四种成分的分析具有高灵敏度；MS在负离子模式下对补骨脂素、异补骨脂查尔酮和补骨脂宁的测定具有高选择性和灵敏度。在对方法进行优化后，通过HPLC结合UV、MS和ECD对[具体物质名称未给出]中的四种成分进行了分离、鉴定和定量的全面测试。
