Yang Jen-Chang, Lee Sheng-Yang, Chen Chi-An, Lin Che-Tong, Chen Chang-Chih, Huang Haw-Ming
Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan.
Bioelectromagnetics. 2010 May;31(4):255-61. doi: 10.1002/bem.20559.
While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF. The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway.
虽然静磁场（SMFs）对成骨细胞分化的影响已得到充分证实，但其机械转导途径仍不清楚。本研究的目的是探讨钙调蛋白在静磁场对成骨细胞的生物物理效应中的作用。将MG63细胞暴露于0.4 T的静磁场中。使用实时聚合酶链反应检测细胞质中磷酸二酯酶RNA的表达。通过检测碱性磷酸酶活性的变化来评估细胞的分化。使用钙调蛋白拮抗剂W-7来评估静磁场模拟后成骨细胞增殖和分化的变化。我们的结果表明，暴露于静磁场可增加MG63细胞中的碱性磷酸酶活性和磷酸二酯酶1C基因表达。添加W-7可显著抑制静磁场诱导的细胞反应。我们认为，静磁场影响成骨细胞成熟的一种可能机制是通过钙调蛋白依赖性机械转导途径。
