Huang Haw-Ming, Lee Sheng-Yang, Yao Wei-Cheng, Lin Che-Tong, Yeh Ching-Ying
Graduate Institute of Oral Sciences, Taipei Medical University, Taipei, Taiwan.
Clin Orthop Relat Res. 2006 Jun;447:201-8. doi: 10.1097/01.blo.0000203464.35561.be.
Cell culture studies have shown that static magnetic fields induce osteoblastic differentiation at an early stage. However, the mechanisms of differentiated effects have not been well described. We postulated that static magnetic fields stimulate osteoblastic differentiation by regulating early local factors released by the cells. To examine our hypothesis, MG63 osteoblast-like cells were exposed continuously to 0.4-T static magnetic fields for 12, 24, 48, and 72 hours. The morphologic changes and matrix vesicles release were observed by scanning and transmission electron microscopy. The effects of static magnetic fields on levels of transforming growth factor-beta1, Type I collagen, osteopontin, and alkaline phosphatase were compared between the exposed and unexposed cells. The data suggest MG63 cells treated with static magnetic fields have more differentiated morphologic features. The local regulatory factors produced by static magnetic field-treated cells were greater than those of the control cultures. These findings provide evidence that static magnetic fields affect osteoblastic maturation by up-regulating early local factors.
细胞培养研究表明，静磁场在早期可诱导成骨细胞分化。然而，其分化作用机制尚未得到充分描述。我们推测，静磁场通过调节细胞释放的早期局部因子来刺激成骨细胞分化。为验证我们的假设，将MG63成骨样细胞连续暴露于0.4-T静磁场中12、24、48和72小时。通过扫描和透射电子显微镜观察形态学变化和基质小泡释放。比较暴露组和未暴露组细胞中静磁场对转化生长因子-β1、I型胶原蛋白、骨桥蛋白和碱性磷酸酶水平的影响。数据表明，经静磁场处理的MG63细胞具有更明显的分化形态特征。经静磁场处理的细胞产生的局部调节因子比对照培养物中的更多。这些发现提供了证据，表明静磁场通过上调早期局部因子来影响成骨细胞成熟。
