Department of Analytical Chemistry, Stockholm University, 106 91 Stockholm, Sweden.
Anal Chem. 2010 Jan 1;82(1):290-6. doi: 10.1021/ac901946b.
A simple platform for combining solid phase extraction (SPE) and surface-assisted laser desorption ionization mass spectrometry (SALDI-MS) of extracted analytes, using disks prepared by embedding graphitized carbon black (GCB-4) particles in a network of polytetrafluoroethylene (PTFE), is presented. The system provides a convenient approach for rapid SALDI-MS screening of substances in aqueous samples, which can be followed by robust quantitative and/or structural analyses by liquid chromatography (LC)/MS/MS of positive samples. The extraction discs are easily transferred between gaskets where the sample extraction and desorption of selected samples is performed and the mass spectrometer. The SPE and SALDI properties of the new GCB-4 disc have been characterized for 15 pesticides with varying chemical properties, and the screening strategy has been applied to the analysis of pesticides in agricultural drainage water. Atrazine and atrazine-desethyl-2-hydroxy were detected in the sampled water by SALDI-MS screening and subsequently confirmed and quantified using LC/MS/MS.
本文提出了一种简单的固相萃取（SPE）和表面辅助激光解吸电离质谱（SALDI-MS）联用平台，用于萃取分析物的分析，该平台使用通过将石墨化炭黑（GCB-4）颗粒嵌入聚四氟乙烯（PTFE）网络中制备的圆盘。该系统提供了一种方便的方法，可用于快速筛选水溶液样品中的物质，然后对阳性样品进行可靠的定量和/或结构分析，通过液相色谱（LC）/MS/MS。萃取盘可轻松在垫圈之间转移，在垫圈中进行样品的萃取和选定样品的解吸，以及质谱仪。对具有不同化学性质的 15 种农药的新型 GCB-4 圆盘的 SPE 和 SALDI 性质进行了表征，并将筛选策略应用于农业排水中农药的分析。通过 SALDI-MS 筛选检测到采样水中的莠去津和莠去津-去乙基-2-羟基，并随后使用 LC/MS/MS 进行确认和定量。
