Choi J H, Lee C H, Yoo K-Y, Kwon S-H, Her S, Shin H-C, Lee Y L, Hwang I K, Lee I S, Won M-H
Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, Republic of South Korea.
J Comp Pathol. 2010 Feb-Apr;142(2-3):147-56. doi: 10.1016/j.jcpa.2009.10.013. Epub 2009 Dec 1.
The immunoreactivity and protein expression of olfactory marker protein (OMP) and tyrosine hydroxylase (TH) in the main olfactory bulb (MOB) of the dog during normal ageing was investigated. OMP immunolabelling was observed only in nerve bundles of the olfactory nerve (ONL) and glomerular layers (GL) and there was no OMP expression within cell bodies of any layer. TH immunolabelling was detected in all layers of the MOB except for the ONL. Most of the neurons expressing TH were distributed in the juxtaglomerular region and had a morphological appearance consistent with periglomerular, external tufted or superficial short axon cells. Dendrites of TH-immunoreactive neurons were closely apposed to OMP-immunoreactive nerve bundles within the glomeruli. There was no significant age-related loss of OMP and TH immunoreactivity and protein concentrations of these molecules were consistent in dogs of different ages. These results suggest that olfactory signal transduction to the GL via axons of olfactory receptor neurons remains unchanged during ageing in the dog.
研究了正常衰老过程中犬主嗅球（MOB）中嗅觉标记蛋白（OMP）和酪氨酸羟化酶（TH）的免疫反应性及蛋白表达。仅在嗅神经（ONL）的神经束和肾小球层（GL）中观察到OMP免疫标记，且任何层的细胞体内均无OMP表达。除ONL外，在MOB的所有层中均检测到TH免疫标记。大多数表达TH的神经元分布在近肾小球区域，其形态外观与肾小球周围、外侧簇状或浅表短轴突细胞一致。TH免疫反应性神经元的树突与肾小球内的OMP免疫反应性神经束紧密相邻。这些分子的OMP和TH免疫反应性及蛋白浓度在不同年龄的犬中无明显的年龄相关性损失且保持一致。这些结果表明，在犬衰老过程中，嗅觉受体神经元轴突向GL的嗅觉信号转导保持不变。
