Department of Gastroenterology, University Hospital Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Pathol Res Pract. 2010 Jan 15;206(1):43-50. doi: 10.1016/j.prp.2009.09.005. Epub 2009 Dec 1.
TNF-related apoptosis inducing-ligand (TRAIL) is a potent inducer of apoptosis and plays an important role in immune regulation. To explore the role of TRAIL in inflammatory bowel disease (IBD), we examined the expression of the TRAIL/TRAIL-receptor system in colonic resections from patients with ulcerative colitis and Crohn's disease in comparison to normal colon and appendicitis. TRAIL and TRAIL-receptor (TRAIL-R) expression was assessed in resections of normal colon, colon of IBD patients, and appendicitis by immunohistochemistry. TRAIL was downregulated in enterocytes of patients with IBD, but was upregulated in mononuclear cells in areas of active mucosal inflammation. For TRAIL-R1, we detected a strong downregulation in the surface epithelium in IBD but not in appendicitis. TRAIL-R2 and TRAIL-R4 were strongly downregulated in the surface epithelium in any kind of mucosal inflammation. TRAIL and TRAIL-R1 are downregulated in enterocytes, and TRAIL is upregulated in mononuclear cells only in IBD but not in normal colon or appendicitis. This may point to a pathophysiologic role of the TRAIL system in inflammatory bowel disease.
肿瘤坏死因子相关凋亡诱导配体（TRAIL）是一种有效的凋亡诱导剂，在免疫调节中发挥重要作用。为了探讨 TRAIL 在炎症性肠病（IBD）中的作用，我们比较了溃疡性结肠炎和克罗恩病患者的结肠切除标本与正常结肠和阑尾炎中 TRAIL/TRAIL 受体系统的表达。通过免疫组织化学法检测正常结肠、IBD 患者结肠和阑尾炎切除标本中 TRAIL 和 TRAIL 受体（TRAIL-R）的表达。TRAIL 在 IBD 患者的肠上皮细胞中下调，但在活跃的黏膜炎症区域的单核细胞中上调。对于 TRAIL-R1，我们在 IBD 中检测到表面上皮的强烈下调，但在阑尾炎中没有。TRAIL-R2 和 TRAIL-R4 在任何类型的黏膜炎症中均在表面上皮中强烈下调。TRAIL 和 TRAIL-R1 在肠上皮细胞中下调，而 TRAIL 仅在 IBD 中的单核细胞中上调，而在正常结肠或阑尾炎中则没有。这可能表明 TRAIL 系统在炎症性肠病中的病理生理作用。
