Laboratório de Entomologia, Faculdade de Veterinária, UFRGS, 91540-000 Porto Alegre, RS, Brazil.
Bioresour Technol. 2010 Apr;101(7):2506-9. doi: 10.1016/j.biortech.2009.11.016. Epub 2009 Dec 1.
Hesperozygis ringens (Benth.) Epling (Lamiaceae) is a strongly aromatic plant employed popularly for its antiparasitic properties. The leaves afforded 4% of essential oil constituted mainly by pulegone (86%). Laboratory tests were carried out to determine the toxicity of the essential oil species on engorged females and larvae of the cattle tick Riphicephalus (Boophilus) microplus using the adult immersion test (AIT) and the larval immersion test (LIT). It was observed that the essential oil at the concentration of 50 microL/mL and 25 microL/mL inhibited the egg laying significatively in relation to the controls and the eggs from these treated females were affected by the oil; the hatching was inhibited in 95% and 30%, respectively. In the LIT it was verified that the LC(99.9), LC(50) and LC(1) was 0.541 microL/mL, 0.260 microL/mL and 0.015 microL/mL, respectively. Pulegone, isolated from the oil, showed similar effect on the females and on the larvae, indicating that it is responsible for the oil activity.
绵穗苏（Benth.）Epling（唇形科）是一种具有强烈香气的植物，因其具有驱虫特性而被广泛使用。叶片提供了 4%的精油，主要由胡薄荷酮（86%）组成。实验室测试使用成虫浸泡试验（AIT）和幼虫浸泡试验（LIT）来确定精油对牛蜱（Boophilus）microplus 饱血雌性和幼虫的毒性。结果表明，精油在 50 微升/毫升和 25 微升/毫升的浓度下，与对照相比，产卵显著受到抑制，并且来自这些处理过的雌虫的卵受到油的影响；孵化分别抑制了 95%和 30%。在 LIT 中，LC（99.9）、LC（50）和 LC（1）分别为 0.541 微升/毫升、0.260 微升/毫升和 0.015 微升/毫升。从油中分离出的胡薄荷酮对雌虫和幼虫表现出相似的作用，表明它是油活性的原因。
